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Product Info Summary
| SKU: | A09224 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MED6 Antibody Picoband®
SKU/Catalog Number
A09224
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MED6 Antibody Picoband® catalog # A09224. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MED6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09224)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MED6 recombinant protein (Position: F40-Q246).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09224 is reactive to MED6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
28.425kDa
Background of MED6
Mediator of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an enzyme that in humans is encoded by the MED6 gene. This family of proteins represent the transcriptional mediator protein subunit 6 that is required for activation of many RNA polymerase II promoters and which are conserved from yeast to humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09224 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A431 whole cell, human Jurkat whole cell, human HepG2 whole cell, human PC-3 whole cell, human U2OS whole cell, human A549 whole cell, human MCF-7 whole cell, rat C6 whole cellmouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MED6 using anti-MED6 antibody (A09224).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: rat C6 whole cell lysates.
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED6 antigen affinity purified polyclonal antibody (Catalog # A09224) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED6 at approximately 34 kDa. The expected band size for MED6 is at 28 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MED6 (Source: Uniprot.org, NCBI)
Gene Name
MED6
Full Name
Mediator of RNA polymerase II transcription subunit 6
Weight
28.425kDa
Superfamily
Mediator complex subunit 6 family
Alternative Names
Mediator of RNA polymerase II transcription subunit 6; Activator-recruited cofactor 33 kDa component; ARC33; Mediator complex subunit 6; hMed6; Renal carcinoma antigen NY-REN-28; MED6; ARC33 MED6 ARC33, NY-REN-28 mediator complex subunit 6 mediator of RNA polymerase II transcription subunit 6|CTD-2540L5.5|activator-recruited cofactor 33 kDa component|renal carcinoma NY-REN-28
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED6, check out the MED6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED6 Antibody Picoband® (A09224)
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Customer Q&As
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4 Customer Q&As for Anti-MED6 Antibody Picoband®
Question
Does A09224 anti-MED6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
As indicated on the product datasheet, A09224 anti-MED6 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-25
Question
Please see the WB image, lot number and protocol we used for intestine using anti-MED6 antibody A09224. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
We are currently using anti-MED6 antibody A09224 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
A. Dhar
Verified customer
Asked: 2018-05-23
Answer
The anti-MED6 antibody (A09224) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-23
Question
Will anti-MED6 antibody A09224 work for ELISA with intestine?
P. Patel
Verified customer
Asked: 2013-02-21
Answer
According to the expression profile of intestine, MED6 is highly expressed in intestine. So, it is likely that anti-MED6 antibody A09224 will work for ELISA with intestine.
Boster Scientific Support
Answered: 2013-02-21
