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Product Info Summary
| SKU: | M04253 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Zebrafish |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-Zebrafish ak2 Antibody (Center)
SKU/Catalog Number
M04253
Size
50 µl
Description
Boster Bio Anti-Zebrafish ak2 Antibody (Center) (Catalog # M04253). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Zebrafish.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Zebrafish ak2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04253)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Zebrafish ak2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 147-183 amino acids from the Central region of Zebrafish ak2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04253 is reactive to ak2 in Human, Mouse, Zebrafish
Reconstitution
Calculated molecular weight
26616 Da
Background of AK2
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04253 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-Zebrafish ak2 Antibody (Center) at 1:2000 dilution
Lane 1: Zebrafish lysates
Lane 2: ZF4 whole cell lysates
Lane 3: human heart lysates
Lane 4: mouse liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 27 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M04253 staining Zebrafish ak2 in zebra fish body tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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M04253 staining Zebrafish ak2 in human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing ZF4 cells stained with M04253 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M04253, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For ak2 (Source: Uniprot.org, NCBI)
Gene Name
ak2
Full Name
Adenylate kinase 2, mitochondrial
Weight
26616 Da
Superfamily
adenylate kinase family
Alternative Names
Adenylate kinase 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03168}, AK 2 {ECO:0000255|HAMAP-Rule:MF_03168}, 2743 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP-AMP transphosphorylase 2 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP:AMP phosphotransferase {ECO:0000255|HAMAP-Rule:MF_03168}, Adenylate monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03168}, ak2 AK2 ADK2 adenylate kinase 2 adenylate kinase 2, mitochondrial|ATP-AMP transphosphorylase 2|ATP:AMP phosphotransferase|adenylate kinase isoenzyme 2, mitochondrial|adenylate monophosphate kinase|testis secretory sperm-binding protein Li 220n
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ak2, check out the ak2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ak2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zebrafish ak2 Antibody (Center) (M04253)
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