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Product Info Summary
| SKU: | A01626-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®
View all Tryptophan Hydroxylase 1/TPH-1 Antibodies
SKU/Catalog Number
A01626-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® catalog # A01626-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01626-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Tryptophan Hydroxylase recombinant protein (Position: M1-K111).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01626-1 is reactive to TPH1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
50.985kDa
Background of Tryptophan Hydroxylase 1/TPH-1
Tryptophan hydroxylase 1 (TPH1) is an isoenzyme of tryptophan hydroxylase which in humans is encoded by the TPH1 gene. This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01626-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human SGC-7901 whole cell, rat heart tissue, mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (A01626-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase antigen affinity purified polyclonal antibody (Catalog # A01626-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.
Protein Target Info & Infographic
Gene/Protein Information For TPH1 (Source: Uniprot.org, NCBI)
Gene Name
TPH1
Full Name
Tryptophan 5-hydroxylase 1
Weight
50.985kDa
Superfamily
biopterin-dependent aromatic amino acid hydroxylase family
Alternative Names
Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH TPH1 TPRH, TRPH tryptophan hydroxylase 1 tryptophan 5-hydroxylase 1|L-tryptophan hydroxylase|indoleacetic acid-5-hydroxylase|tryptophan 5-monooxygenase 1|tryptophan hydroxylase (tryptophan 5-monooxygenase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPH1, check out the TPH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband® (A01626-1)
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1 Customer Q&As for Anti-Tryptophan Hydroxylase/TPH1 Antibody Picoband®
Question
We are currently using anti-Tryptophan Hydroxylase/TPH1 antibody A01626-1 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
A. Lewis
Verified customer
Asked: 2015-12-31
Answer
The anti-Tryptophan Hydroxylase/TPH1 antibody (A01626-1) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-31
