Product Info Summary
SKU: | PB9236 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband®
SKU/Catalog Number
PB9236
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® catalog # PB9236. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9236)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9236 is reactive to ZEB2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
200 kDa
Calculated molecular weight
136447 MW
Background of ZEB2
ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9236 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human HEL whole cell
IHC: human tonsil tissue, mouse spleen tissue, rat spleen tissueFCM: U937 cell, THP-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ZEB2 using anti-ZEB2 antibody (PB9236).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZEB2 antigen affinity purified polyclonal antibody (Catalog # PB9236) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZEB2 at approximately 200 kDa. The expected band size for ZEB2 is at 200 kDa.
Click image to see more details
Figure 3. Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236).
Overlay histogram showing U937 cells stained with PB9236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 4. Flow Cytometry analysis of THP-1 cells using anti-ZEB2 antibody (PB9236).
Overlay histogram showing THP-1 cells stained with PB9236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 5. IHC analysis of ZEB2 using anti-ZEB2 antibody (PB9236).
ZEB2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZEB2 Antibody (PB9236) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of ZEB2 using anti-ZEB2 antibody (PB9236).
ZEB2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZEB2 Antibody (PB9236) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of ZEB2 using anti-ZEB2 antibody (PB9236).
ZEB2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZEB2 Antibody (PB9236) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ZEB2 (Source: Uniprot.org, NCBI)
Gene Name
ZEB2
Full Name
Zinc finger E-box-binding homeobox 2
Weight
136447 MW
Superfamily
delta-EF1/ZFH-1 C2H2-type zinc-finger family
Alternative Names
Zinc finger E-box-binding homeobox 2;Smad-interacting protein 1;SMADIP1;Zinc finger homeobox protein 1b;ZEB2;KIAA0569, SIP1, ZFHX1B, ZFX1B;HRIHFB2411; ZEB2 HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B zinc finger E-box binding homeobox 2 zinc finger E-box-binding homeobox 2|SMAD interacting protein 1|Smad-interacting protein 1|zinc finger homeobox 1b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZEB2, check out the ZEB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZEB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® (PB9236)
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Customer Q&As
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5 Customer Q&As for Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband®
Question
We ordered your anti-Smad Interacting Protein 1/ZEB2 antibody for IHC-F on corpus callosum in the past. I am using human, and We want to use the antibody for WB next. We need examining corpus callosum as well as fetal brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
I have checked the website and datasheets of our anti-Smad Interacting Protein 1/ZEB2 antibody and it seems that PB9236 has been validated on human in both IHC-F and WB. Thus PB9236 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-20
Question
My colleagues were satisfied with the WB result of your anti-Smad Interacting Protein 1/ZEB2 antibody. However we have observed positive staining in liver nucleus using this antibody. Is that expected? Could you tell me where is ZEB2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
From literature, liver does express ZEB2. Generally ZEB2 expresses in nucleus. Regarding which tissues have ZEB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 14702039
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 9853615
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-10-31
Question
We are currently using anti-Smad Interacting Protein 1/ZEB2 antibody PB9236 for human tissue, and we are happy with the IHC-F results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
The anti-Smad Interacting Protein 1/ZEB2 antibody (PB9236) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-16
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-Smad Interacting Protein 1/ZEB2 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
According to literature liver does express ZEB2. According to Uniprot.org, ZEB2 is expressed in corpus callosum, brain, fetal brain, erythroleukemia, liver, among other tissues. Regarding which tissues have ZEB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 14702039
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 9853615
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-06-12
Question
Our lab want to know about using your anti-Smad Interacting Protein 1/ZEB2 antibody for corticospinal tract morphogenesis studies. Has this antibody been tested with western blotting on smmc whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
We appreciate your inquiry. This PB9236 anti-Smad Interacting Protein 1/ZEB2 antibody is tested on smmc whole cell lysate, u937 cells. It is guaranteed to work for Flow Cytometry, IHC-P, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-15