Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband®

ZEB2 antibody

Boster Bio Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® catalog # PB9236. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9236
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, IHC-F, ICC, WB

Product Name

Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband®

View all ZEB2 Antibodies

SKU/Catalog Number

PB9236

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® catalog # PB9236. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9236)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9236 is reactive to ZEB2 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

200 kDa

Calculated molecular weight

136447 MW

Background of ZEB2

ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1 or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, this gene is mapped to 2q22. It has been found that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines, and the expression of SNAI1 in epithelial cells can trigger an epithelial-mesenchyme transition.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9236 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human HEL whole cell
IHC: human tonsil tissue, mouse spleen tissue, rat spleen tissueFCM: U937 cell, THP-1 cell

Validation Images & Assay Conditions

Gene/Protein Information For ZEB2 (Source: Uniprot.org, NCBI)

Gene Name

ZEB2

Full Name

Zinc finger E-box-binding homeobox 2

Weight

136447 MW

Superfamily

delta-EF1/ZFH-1 C2H2-type zinc-finger family

Alternative Names

Zinc finger E-box-binding homeobox 2;Smad-interacting protein 1;SMADIP1;Zinc finger homeobox protein 1b;ZEB2;KIAA0569, SIP1, ZFHX1B, ZFX1B;HRIHFB2411; ZEB2 HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B zinc finger E-box binding homeobox 2 zinc finger E-box-binding homeobox 2|SMAD interacting protein 1|Smad-interacting protein 1|zinc finger homeobox 1b

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ZEB2, check out the ZEB2 Infographic

ZEB2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ZEB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Smad Interacting Protein 1/ZEB2 Antibody Picoband®

Question

We ordered your anti-Smad Interacting Protein 1/ZEB2 antibody for IHC-F on corpus callosum in the past. I am using human, and We want to use the antibody for WB next. We need examining corpus callosum as well as fetal brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

I have checked the website and datasheets of our anti-Smad Interacting Protein 1/ZEB2 antibody and it seems that PB9236 has been validated on human in both IHC-F and WB. Thus PB9236 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-20

Question

My colleagues were satisfied with the WB result of your anti-Smad Interacting Protein 1/ZEB2 antibody. However we have observed positive staining in liver nucleus using this antibody. Is that expected? Could you tell me where is ZEB2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

From literature, liver does express ZEB2. Generally ZEB2 expresses in nucleus. Regarding which tissues have ZEB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 14702039
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 9853615
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-10-31

Question

We are currently using anti-Smad Interacting Protein 1/ZEB2 antibody PB9236 for human tissue, and we are happy with the IHC-F results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

The anti-Smad Interacting Protein 1/ZEB2 antibody (PB9236) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-16

Question

We have seen staining in human liver. Do you have any suggestions? Is anti-Smad Interacting Protein 1/ZEB2 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

According to literature liver does express ZEB2. According to Uniprot.org, ZEB2 is expressed in corpus callosum, brain, fetal brain, erythroleukemia, liver, among other tissues. Regarding which tissues have ZEB2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9628581, 14702039
Erythroleukemia, Pubmed ID: 23186163
Fetal brain, Pubmed ID: 9853615
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-06-12

Question

Our lab want to know about using your anti-Smad Interacting Protein 1/ZEB2 antibody for corticospinal tract morphogenesis studies. Has this antibody been tested with western blotting on smmc whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

We appreciate your inquiry. This PB9236 anti-Smad Interacting Protein 1/ZEB2 antibody is tested on smmc whole cell lysate, u937 cells. It is guaranteed to work for Flow Cytometry, IHC-P, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-08-15

Order DetailsPrice
PB9236

100μg

$370
PB9236-10ug

10μg sample (liquid)

$99
PB9236-Biotin

100 μg Biotin conjugated

$570
PB9236-Cy3

100 μg Cy3 conjugated

$570
PB9236-Dylight488

100 μg Dylight488 conjugated

$570
PB9236-Dylight550

100 μg Dylight550 conjugated

$570
PB9236-Dylight594

100 μg Dylight594 conjugated

$570
PB9236-FITC

100 μg FITC conjugated

$570
PB9236-HRP

100 μg HRP conjugated

$570
PB9236-APC

100 μg APC conjugated

$670
PB9236-PE

100 μg PE conjugated

$670
PB9236-iFluor647

100 μg iFluor647 conjugated

$670
PB9236-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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