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Product Info Summary
| SKU: | EK2071 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Rat GFRA1 ELISA Kit PicoKine®
View all GFR alpha-1/GDNF R alpha-1 ELISA kits
SKU/Catalog Number
EK2071
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat GFRA1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gfra1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat GFRA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2071)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D25-S430
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2071 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2071 is reactive to Gfra1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2071 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GFR alpha-1/GDNF R alpha-1
GDNF family receptor alpha-1 (GFRα1), also known as the GDNF receptor, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to 1q55. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.GDNF family receptor alpha 1 has been shown to interact with GDNF[8][9] and RET proto-oncogene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2071-CAP | Anti-Rat Gfra1 Pre-coated 96-well strip microplate | 1 |
| EK2071-ST | Rat Gfra1 Standard | 2 vials, 10 ng/tube |
| EK2071-DA | Rat Gfra1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.039 | 0.130 | 0.242 | 0.507 | 0.824 | 1.311 | 1.776 | 2.194 |
Data Example Images
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Rat GFRA1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GFR alpha-1/GDNF R alpha-1, Dilution ratio of 1:4, concentration in serum and plasma is around 1500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat GFRA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 78 | 249 | 857 | 81 | 254 | 955 |
| Standard deviation | 3.20 | 11.45 | 45.42 | 4.46 | 17.02 | 71.63 |
| CV (%) | 4.1% | 4.6% | 5.3% | 5.5% | 6.7% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GFR alpha-1/GDNF R alpha-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 22 | 21 | 21 | 23 | 21 | 0.82 | 3.9% |
| Sample 2 | 99 | 94 | 88 | 91 | 93 | 4.06 | 4.3% |
| Sample 3 | 314 | 347 | 308 | 295 | 316 | 19.17 | 6.0% |
Protein Target Info & Infographic
Gene/Protein Information For Gfra1 (Source: Uniprot.Org, NCBI)
Gene Name
Gfra1
Full Name
GDNF family receptor alpha-1
Weight
51.456kDa
Superfamily
GDNFR family
Alternative Names
GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1; Gfra1; Gdnfra; Retl1; Trnr1 GFRA1 GDNFR, GDNFRA, GFR-ALPHA-1, GFRalpha-1, RET1L, RETL1, TRNR1 GDNF family receptor alpha 1 GDNF family receptor alpha-1|GDNF receptor alpha 1d|GDNF receptor alpha 1e|GDNFR-alpha-1|GPI-linked anchor protein|Glial cell line-derived neurotrophic factor receptor alpha|PI-linked cell-surface accessory protein|RET ligand 1|TGF-beta-related neurotrophic factor receptor 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Gfra1, check out the Gfra1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Gfra1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat GFRA1 ELISA Kit PicoKine® (EK2071)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Rat GFRA1 ELISA Kit PicoKine®
Question
Q: can I use heparin plasma as samples in Rat GFR alpha-1 Picokine® ELISA Kit (Catalog # EK2071)?
Verified Customer
Verified customer
Asked: 2020-06-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of GFR alpha-1 causing disruption of its protein structure. GFR alpha-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the GFR alpha-1 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-26
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
M. Wu
Verified customer
Asked: 2019-11-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-11-27
Question
Q: Can GFR alpha-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-07-31
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GFR alpha-1 using the EK2071 Rat GFR alpha-1 Picokine® ELISA Kit?
J. Krishna
Verified customer
Asked: 2019-05-15
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-05-15
Question
Q: how to thaw whole blood sample for GFR alpha-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-02-03
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GFR alpha-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-02-03
Question
Q: What is the optimal O.D. value for GFR alpha-1 ELISA kit? I performed your GFR alpha-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain GFR alpha-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-12-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GFR alpha-1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of GFR alpha-1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-12-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Moore
Verified customer
Asked: 2018-10-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-06
Question
Q: how to proceed with the analysis of ELISA data? I measured GFR alpha-1 level in plasma.
Verified Customer
Verified customer
Asked: 2018-03-18
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-03-18
