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Product Info Summary
| SKU: | A01169 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ZEB2 Antibody
SKU/Catalog Number
A01169
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZEB2 Antibody (Catalog # A01169). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZEB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZEB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01169)
Host
Rabbit
Contents
ZEB2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZEB2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ZEB2. The immunogen is located within the last 50 amino acids of ZEB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ZEB2 antibody is predicted to not cross-react with ZEB1.
Reactive Species
A01169 is reactive to ZEB2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
136447 MW
Background of ZEB2
ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01169 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZEB2 antibody can be used for detection of ZEB2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZEB2 in EL4 cell lysate with ZEB2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.
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Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/ml.
Red: ZEB2 Antibody (A01169)
Blue: DAPI staining
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Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ZEB2 (Source: Uniprot.org, NCBI)
Gene Name
ZEB2
Full Name
Zinc finger E-box-binding homeobox 2
Weight
136447 MW
Superfamily
delta-EF1/ZFH-1 C2H2-type zinc-finger family
Alternative Names
SIP1, SIP-1, ZFHX1B, HSPC082, SMADIP1, KIAA0569, SIP1, ZFX1B, HRIHFB2411, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1 ZEB2 HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B zinc finger E-box binding homeobox 2 zinc finger E-box-binding homeobox 2|SMAD interacting protein 1|Smad-interacting protein 1|zinc finger homeobox 1b
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZEB2, check out the ZEB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZEB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZEB2 Antibody (A01169)
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