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Product Info Summary
| SKU: | PB9968 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ZAP70 Antibody Picoband®
SKU/Catalog Number
PB9968
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZAP70 Antibody Picoband® catalog # PB9968. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZAP70 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9968)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ZAP70, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9968 is reactive to ZAP70 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
69872 MW
Background of ZAP70
ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9968 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human Jurkat whole cell, human CCRF-CEM whole cell, human MOLT-4 whole cell, human Raji whole cell, rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue
IHC: human prostate cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ZAP70 using anti-ZAP70 antibody (PB9968).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human CCRF-CEM whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZAP70 antigen affinity purified polyclonal antibody (Catalog # PB9968) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZAP70 at approximately 70 kDa. The expected band size for ZAP70 is at 70 kDa.
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Figure 2. IHC analysis of ZAP70 using anti-ZAP70 antibody (PB9968).
ZAP70 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZAP70 Antibody (PB9968) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ZAP70 (Source: Uniprot.org, NCBI)
Gene Name
ZAP70
Full Name
Tyrosine-protein kinase ZAP-70
Weight
69872 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase ZAP-70;2.7.10.2;70 kDa zeta-chain associated protein;Syk-related tyrosine kinase;ZAP70;SRK; ZAP70 ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70 zeta chain of T cell receptor associated protein kinase 70 tyrosine-protein kinase ZAP-70|70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|zeta chain of T cell receptor associated protein kinase 70kDa|zeta-chain (TCR) associated protein kinase 70kDa|zeta-chain associated protein kinase, 70kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZAP70, check out the ZAP70 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZAP70: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZAP70 Antibody Picoband® (PB9968)
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Customer Q&As
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1 Customer Q&As for Anti-ZAP70 Antibody Picoband®
Question
We are currently using anti-ZAP70 antibody PB9968 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
The anti-ZAP70 antibody (PB9968) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-28
