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Product Info Summary
| SKU: | M00833 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-YY1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00833
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-YY1 Rabbit Monoclonal Antibody catalog # M00833. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-YY1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00833)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AIE-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human YY1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00833 is reactive to YY1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
44713 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00833 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:3000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50-1:100
Positive Control
WB: human Hela whole cell, human CACO-2 whole cell, human MCF-7 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse RAW2647 whole cell, mouse ANA-1 whole cell
IHC: human bladder using YY1 Antibody
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of YY1 using anti-YY1 antibody (M00833).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YY1 antigen affinity purified monoclonal antibody (Catalog # M00833) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YY1 at approximately 68 kDa. The expected band size for YY1 is at 45 kDa.
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Immunohistochemical analysis of paraffin-embedded human bladder using YY1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis of Hela cells, using YY1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For YY1 (Source: Uniprot.org, NCBI)
Gene Name
YY1
Full Name
Transcriptional repressor protein YY1
Weight
44713 MW
Superfamily
YY transcription factor family
Alternative Names
Transcriptional repressor protein YY1;Delta transcription factor;INO80 complex subunit S;NF-E1;Yin and yang 1;YY-1;YY1;INO80S; YY1 DELTA, GADEVS, INO80S, NF-E1, UCRBP, YIN-YANG-1 YY1 transcription factor transcriptional repressor protein YY1|INO80 complex subunit S|YY-1|Yin and Yang 1 protein|delta transcription factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YY1, check out the YY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-YY1 Rabbit Monoclonal Antibody (M00833)
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