Click image to see more details
Product Info Summary
| SKU: | PA2195 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Leupaxin/LPXN Antibody Picoband®
SKU/Catalog Number
PA2195
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leupaxin/LPXN Antibody catalog # PA2195. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leupaxin/LPXN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2195)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Leupaxin, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2195 is reactive to LPXN in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
43332 MW
Background of Leupaxin
Leupaxin is a protein that in humans is encoded by the LPXN gene. This gene is mapped to 11q12.1. The product encoded by this gene is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2195 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Raji whole cell, human PC-3 whole cell, human U87 whole cell, human MDA-MB-453 whole cell, mouse RAW2647 whole cell
FCM: Raji cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LPXN using anti-LPXN antibody (PA2195).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human MDA-MB-453 whole cell lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPXN antigen affinity purified polyclonal antibody (Catalog # PA2195) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPXN at approximately 43 kDa. The expected band size for LPXN is at 43 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of Raji cells using anti-LPXN antibody (PA2195).
Overlay histogram showing Raji cells stained with PA2195 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LPXN Antibody (PA2195, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For LPXN (Source: Uniprot.org, NCBI)
Gene Name
LPXN
Full Name
Leupaxin
Weight
43332 MW
Superfamily
paxillin family
Alternative Names
Leupaxin;LPXN;LDLP; LPXN LDPL leupaxin leupaxin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LPXN, check out the LPXN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LPXN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leupaxin/LPXN Antibody Picoband® (PA2195)
Hello CJ!
No publications found for PA2195
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Leupaxin/LPXN Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Leupaxin/LPXN Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Leupaxin/LPXN Antibody Picoband®
Question
Is this PA2195 anti-Leupaxin/LPXN antibody reactive to the isotypes of LPXN?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The immunogen of PA2195 anti-Leupaxin/LPXN antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Leupaxin(115-129aa KKHLPDKQDHKASLD), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-28
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Leupaxin/LPXN antibody PA2195. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
Can you help my question with product PA2195, anti-Leupaxin/LPXN antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2195 anti-Leupaxin/LPXN antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-12
Question
We are currently using anti-Leupaxin/LPXN antibody PA2195 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
E. Moore
Verified customer
Asked: 2019-04-18
Answer
The anti-Leupaxin/LPXN antibody (PA2195) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-18
Question
you antibody to test anti-Leupaxin/LPXN antibody PA2195 on rat liver for research purposes, then I may be interested in using anti-Leupaxin/LPXN antibody PA2195 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Carter
Verified customer
Asked: 2015-06-05
Answer
The products we sell, including anti-Leupaxin/LPXN antibody PA2195, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-06-05
