Anti-YWHAE Antibody Picoband®

14-3-3 epsilon antibody

Boster Bio Anti-YWHAE Antibody Picoband® catalog # A01687-5. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A01687-5
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC, ICC, WB

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Product Name

Anti-YWHAE Antibody Picoband®

View all 14-3-3 epsilon Antibodies

SKU/Catalog Number

A01687-5

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-YWHAE Antibody Picoband® catalog # A01687-5. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-YWHAE Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01687-5)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human YWHAE recombinant protein (Position: Q16-L163).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01687-5 is reactive to YWHAE in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

32 kDa

Calculated molecular weight

29.174kDa

Background of 14-3-3 epsilon

14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01687-5 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml/ml, Human

Positive Control

WB: human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, human 293T whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: human lung cancer tissue, human prostate cancer tissue, mouse brain tissue, rat brain tissue
ICC/IF: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For YWHAE (Source: Uniprot.org, NCBI)

Gene Name

YWHAE

Full Name

14-3-3 protein epsilon

Weight

29.174kDa

Superfamily

14-3-3 family

Alternative Names

Periaxin; PRX; KIAA1620 YWHAE 14-3-3E, HEL2, KCIP-1, MDCR, MDS tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon 14-3-3 protein epsilon|14-3-3 epsilon|epididymis luminal protein 2|mitochondrial import stimulation factor L subunit|protein kinase C inhibitor protein-1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide|tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on YWHAE, check out the YWHAE Infographic

YWHAE infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for YWHAE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A01687-5

100μg

$370
A01687-5-10ug

10μg sample (liquid)

$99
A01687-5-Biotin

100 μg Biotin conjugated

$570
A01687-5-Cy3

100 μg Cy3 conjugated

$570
A01687-5-Dylight488

100 μg Dylight488 conjugated

$570
A01687-5-Dylight550

100 μg Dylight550 conjugated

$570
A01687-5-Dylight594

100 μg Dylight594 conjugated

$570
A01687-5-FITC

100 μg FITC conjugated

$570
A01687-5-HRP

100 μg HRP conjugated

$570
A01687-5-APC

100 μg APC conjugated

$670
A01687-5-PE

100 μg PE conjugated

$670
A01687-5-iFluor647

100 μg iFluor647 conjugated

$670
A01687-5-carrier-free

Carrier Free

$370

More conjugates/formats

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A01687-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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