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Product Info Summary
| SKU: | A09853 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-YTHDF1 Antibody
SKU/Catalog Number
A09853
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-YTHDF1 Antibody (Catalog # A09853). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-YTHDF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09853)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human YTHDF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09853 is reactive to YTHDF1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
60874 MW
Background of YTHD1
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
Ota T., Nat. Genet. 36:40-45(2004).
Deloukas P., Nature 414:865-871(2001).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09853 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using YTHDF1 antibody A09853.
Protein Target Info & Infographic
Gene/Protein Information For YTHDF1 (Source: Uniprot.org, NCBI)
Gene Name
YTHDF1
Full Name
YTH domain-containing family protein 1
Weight
60874 MW
Alternative Names
YTH domain-containing family protein 1 ;Dermatomyositis associated with cancer putative autoantigen 1 ;DACA-1 ;YTHDF1 ;C20orf21 ; YTHDF1 C20orf21 YTH N6-methyladenosine RNA binding protein 1 YTH domain-containing family protein 1|DACA-1|YTH N(6)-methyladenosine RNA binding protein 1|YTH domain family 1|YTH domain family protein 1|YTH domain family, member 1|dermatomyositis associated with cancer putative auto 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YTHDF1, check out the YTHDF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YTHDF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-YTHDF1 Antibody (A09853)
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No publications found for A09853
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Customer Q&As
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4 Customer Q&As for Anti-YTHDF1 Antibody
Question
Is this A09853 anti-YTHDF1 antibody reactive to the isotypes of YTHDF1?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The immunogen of A09853 anti-YTHDF1 antibody is Synthesized peptide derived from N-terminal of human YTHDF1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-10
Question
We are currently using anti-YTHDF1 antibody A09853 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
The anti-YTHDF1 antibody (A09853) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-27
Question
My lab would like to test anti-YTHDF1 antibody A09853 on rat ileal mucosa for research purposes, then I may be interested in using anti-YTHDF1 antibody A09853 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Banerjee
Verified customer
Asked: 2016-06-08
Answer
The products we sell, including anti-YTHDF1 antibody A09853, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-06-08
Question
Can you help my question with product A09853, anti-YTHDF1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Taylor
Verified customer
Asked: 2014-05-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09853 anti-YTHDF1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-05-02
