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Product Info Summary
| SKU: | A01275T714 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-XRCC5 (phospho-T714) Antibody
View all Ku80/XRCC5 Antibodies
SKU/Catalog Number
A01275T714
Size
100ul
Form
Liquid
Description
Boster Bio Anti-XRCC5 (phospho-T714) Antibody catalog # A01275T714. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XRCC5 (phospho-T714) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01275T714)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human XRCC5 around the phosphorylation site of Threonine 714.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01275T714 is reactive to XRCC5 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
82705 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01275T714 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of XRCC5 using Anti-XRCC5 (phospho-T714) Antibody (A01275T714).
Immunohistochemistry (IHC) analyzes of p-Ku-80 (T714) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-XRCC5 (phospho-T714) Antibody A01275T714
Western blot (WB) analysis of p-XRCC5 (T714) pAb at 1:500 dilutionLane1:A549 whole cell lysate(40ug)
Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug)
Lane3:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug)
Lane4:The Brain tissue lysate of Mouse(40ug)
Lane5:The Uterus tissue lysate of Rat(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For XRCC5 (Source: Uniprot.org, NCBI)
Gene Name
XRCC5
Full Name
X-ray repair cross-complementing protein 5
Weight
82705 MW
Superfamily
ku80 family
Alternative Names
X-ray repair cross-complementing protein 5;3.6.4.-;86 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 2;ATP-dependent DNA helicase II 80 kDa subunit;CTC box-binding factor 85 kDa subunit;CTC85;CTCBF;DNA repair protein XRCC5;Ku80;Ku86;Lupus Ku autoantigen protein p86;Nuclear factor IV;Thyroid-lupus autoantigen;TLAA;X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining);XRCC5;G22P2; XRCC5 KARP-1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair cross complementing 5 X-ray repair cross-complementing protein 5|86 kDa subunit of Ku |ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku auto, 80kDa|Ku86 auto related protein 1|TLAA|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|lupus Ku auto protein p86|nuclear factor IV|thyroid-lupus auto
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XRCC5, check out the XRCC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XRCC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XRCC5 (phospho-T714) Antibody (A01275T714)
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