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Product Info Summary
| SKU: | A02138-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-XRCC2 Antibody Picoband®
SKU/Catalog Number
A02138-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-XRCC2 Antibody Picoband® catalog # A02138-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XRCC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02138-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human XRCC2 recombinant protein (Position: A4-E106).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02138-3 is reactive to XRCC2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
32 kDa
Calculated molecular weight
31.956kDa
Background of XRCC2
DNA repair protein XRCC2 is a protein that in humans is encoded by the XRCC2 gene.This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02138-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HEK293 whole cell, human K562 whole cell, rat kidney tissue, mouse SP2/0 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of XRCC2 using anti-XRCC2 antibody (A02138-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: mouse SP2/0 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC2 antigen affinity purified polyclonal antibody (Catalog # A02138-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC2 at approximately 32KD. The expected band size for XRCC2 is at 32KD.
Protein Target Info & Infographic
Gene/Protein Information For XRCC2 (Source: Uniprot.org, NCBI)
Gene Name
XRCC2
Full Name
DNA repair protein XRCC2
Weight
31.956kDa
Superfamily
RecA family
Alternative Names
DNA repair protein XRCC2; X-ray repair cross-complementing protein 2; Xrcc2 XRCC2 FANCU, POF17, SPGF50 X-ray repair cross complementing 2 DNA repair protein XRCC2|X-ray repair complementing defective repair in Chinese hamster cells 2|X-ray repair cross-complementing protein 2|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XRCC2, check out the XRCC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XRCC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-XRCC2 Antibody Picoband® (A02138-3)
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