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Product Info Summary
| SKU: | PA1985 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Protein Wnt-2b Wnt2b Antibody Picoband®
SKU/Catalog Number
PA1985
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein Wnt-2b Wnt2b Antibody catalog # PA1985. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein Wnt-2b Wnt2b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1985)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Wnt2b, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1985 is reactive to WNT2B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
450 kDa
Calculated molecular weight
43770 MW
Background of Wnt-2b
WNT2B (Wingless-Type MMTV Integration Site Family Member 2B), is a protein that in humans is encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. Katoh et al. (1996) used fluorescence in situ hybridization to map the WNT13 gene to chromosome 1p13. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1985 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Anti-Wnt2b antibody, PA1985, Western blotting
Recombinant Protein Detection Source :E.coli derived -recombinant Human WNT2B, 35.78KD
(162aa tag+ R101-A254)
Lane 1: Recombinant Human WNT2B Protein 10ng
Lane 2: Recombinant Human WNT2B Protein 5ng
Lane 3: Recombinant Human WNT2B Protein 2.5ng
Protein Target Info & Infographic
Gene/Protein Information For WNT2B (Source: Uniprot.org, NCBI)
Gene Name
WNT2B
Full Name
Protein Wnt-2b
Weight
43770 MW
Superfamily
Wnt family
Alternative Names
Protein Wnt-2b;Protein Wnt-13;WNT2B;WNT13; WNT2B WNT13 Wnt family member 2B protein Wnt-2b|XWNT2, Xenopus, homolog of|wingless-type MMTV integration site family, member 13|wingless-type MMTV integration site family, member 2B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WNT2B, check out the WNT2B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WNT2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein Wnt-2b Wnt2b Antibody Picoband® (PA1985)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Protein Wnt-2b Wnt2b Antibody Picoband®
Question
Would anti-Wnt2b antibody PA1985 work for WB with milk?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
According to the expression profile of milk, WNT2B is highly expressed in milk. So, it is likely that anti-Wnt2b antibody PA1985 will work for WB with milk.
Boster Scientific Support
Answered: 2020-04-21
Question
Will PA1985 anti-Wnt2b antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
It shows on the product datasheet, PA1985 anti-Wnt2b antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-03
Question
Is this PA1985 anti-Wnt2b antibody reactive to the isotypes of WNT2B?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
The immunogen of PA1985 anti-Wnt2b antibody is A synthetic peptide corresponding to a sequence in the middle region of human Wnt2b(169-185aa DPYTRGRHHDQRGDFDW), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-24
Question
Please see the WB image, lot number and protocol we used for milk using anti-Wnt2b antibody PA1985. Please let me know if you require anything else.
D. Johnson
Verified customer
Asked: 2015-06-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-06-02
