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Product Info Summary
| SKU: | PB9238 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC, WB |
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Product info
Product Name
Anti-IL16 Antibody Picoband®
SKU/Catalog Number
PB9238
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL16 Antibody Picoband® catalog # PB9238. Tested in ELISA, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9238)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IL-16 recombinant protein (Position: S1212-S1332). Human IL-16 shares 86% amino acid (aa) sequence identity with mouse IL-16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9238 is reactive to IL16 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45-55 kDa
Calculated molecular weight
141752 MW
Background of IL-16
Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. It is mapped to 15q25.1. IL-16 was originally described as a factor that could attract activated T cells in humans. It was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16 stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9238 is guaranteed for ELISA, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U937 whole cell
IHC: Human Tonsil Tissue
ICC: human cord blood
Validation Images & Assay Conditions
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Anti-IL-16 antibody, PB9238, Western blotting
All lanes: Anti IL-16 (PB9238) at 0.5ug/ml
WB: Recombinant Human IL-16 Protein 0.5ng
Predicted bind size: 17KD
Observed bind size: 17KD
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Anti-IL-16 antibody, PB9238, IHC(P)
IHC(P): Human Tonsil Tissue
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Figure 3. IHC analysis of IL16 using anti-IL16 antibody (PB9238).
IL16 was detected in immunocytochemical section of human cord blood. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-IL16 Antibody (PB9238) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. Western blot analysis of IL16 using anti-IL16 antibody (PB9238).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U937 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL16 antigen affinity purified polyclonal antibody (Catalog # PB9238) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL16 at approximately 45-55KD. The expected band size for IL16 is at 142KD.
Protein Target Info & Infographic
Gene/Protein Information For IL16 (Source: Uniprot.org, NCBI)
Gene Name
IL16
Full Name
Pro-interleukin-16
Weight
141752 MW
Alternative Names
Pro-interleukin-16;Interleukin-16;IL-16;Lymphocyte chemoattractant factor;LCF;IL16; IL16 LCF, NIL16, PRIL16, prIL-16 interleukin 16 pro-interleukin-16|lymphocyte chemoattractant factor|neuronal interleukin 16|prointerleukin 16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL16, check out the IL16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL16 Antibody Picoband® (PB9238)
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1 Customer Q&As for Anti-IL16 Antibody Picoband®
Question
We are currently using anti-IL16 antibody PB9238 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
The anti-IL16 antibody (PB9238) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-06
