Product Info Summary
SKU: | M02574 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Wnt-10b Monoclonal Antibody
SKU/Catalog Number
M02574
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Wnt-10b Monoclonal Antibody catalog # M02574. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt-10b Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02574)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5A7
Isotype
IgG
Immunogen
Purified recombinant fragment of human Wnt-10b expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02574 is reactive to WNT10B in Human
Reconstitution
Calculated molecular weight
43000 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02574 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Immunofluorescent staining data of WNT10B using Anti-Wnt-10b Monoclonal Antibody (M02574).
Confocal Immunofluorescence (IF) analysis of PANC-1 cells using Wnt-10b Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Click image to see more details
Figure 2. Immunohistochemistry validation of WNT10B using Anti-Wnt-10b Monoclonal Antibody (M02574).
Immunohistochemistry (IHC) analysis of paraffin-embedded human normal stomach (A)Click image to see more details
Figure 1. Western blotting validation for Anti-Wnt-10b Monoclonal Antibody M02574
Western Blot (WB) analysis using Wnt-10b Monoclonal Antibody against HeLa cell lysate (1).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For WNT10B (Source: Uniprot.org, NCBI)
Gene Name
WNT10B
Full Name
Protein Wnt-10b
Weight
43000 MW
Superfamily
Wnt family
Alternative Names
Protein Wnt-10b;Protein Wnt-12;WNT10B;WNT12; WNT10B SHFM6, STHAG8, WNT-12 Wnt family member 10B protein Wnt-10b|WNT-10B protein|wingless-type MMTV integration site family, member 10B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WNT10B, check out the WNT10B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WNT10B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Wnt-10b Monoclonal Antibody (M02574)
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No publications found for M02574
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