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Product Info Summary
| SKU: | A02574 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Protein Wnt-10b Wnt10b Antibody
SKU/Catalog Number
A02574
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein Wnt-10b Wnt10b Antibody (Catalog # A02574). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Wnt10b antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein Wnt-10b Wnt10b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02574)
Host
Rabbit
Contents
Wnt10b Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b. The immunogen is located within amino acids 130 - 180 of Wnt10b.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.
Reactive Species
A02574 is reactive to WNT10B in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
43000 MW
Background of Wnt-10b
Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02574 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Wnt10b in human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For WNT10B (Source: Uniprot.org, NCBI)
Gene Name
WNT10B
Full Name
Protein Wnt-10b
Weight
43000 MW
Superfamily
Wnt family
Alternative Names
SHFM6, WNT-12, WNT12, Protein Wnt-10b, Protein Wnt-12 WNT10B SHFM6, STHAG8, WNT-12 Wnt family member 10B protein Wnt-10b|WNT-10B protein|wingless-type MMTV integration site family, member 10B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WNT10B, check out the WNT10B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WNT10B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein Wnt-10b Wnt10b Antibody (A02574)
Hello CJ!
A02574 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
An R,Wang X,Yang L,Zhang J,Wang N,Xu F,Hou Y,Zhang H,Zhang L. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats.Toxicology.2020 Dec 24;449:152665. doi:10.1016/j.tox.2020.152665.Epub ahead of p
Species: Rat
A02574 usage in article: APP:WB, SAMPLE:OVARY TISSUE, DILUTION:1:500
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