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Product Info Summary
| SKU: | A00199 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Wilms tumor protein WT1 Antibody
SKU/Catalog Number
A00199
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Wilms tumor protein WT1 Antibody catalog # A00199. Tested in WB,IF,IHC,ELISA applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wilms tumor protein WT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00199)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLH conjugated synthetic peptide derived from human WT1: 301-400/449
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00199 is reactive to WT1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
49188 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00199 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:100-1:500
IF: 1:100-1:500
ELISA: 1:5000-1:10000
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of WT1 using Anti-Wilms tumor protein WT1 Antibody (A00199).
ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Wilms tumor protein WT1 Antibody A00199
Western blot analysis of Wilms Tumor Protein on human kidney lysates using anti-Wilms Tumor Protein antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For WT1 (Source: Uniprot.org, NCBI)
Gene Name
WT1
Full Name
Wilms tumor protein
Weight
49188 MW
Superfamily
EGR C2H2-type zinc-finger protein family
Alternative Names
Wilms tumor protein;WT33;WT1; WT1 AWT1, GUD, NPHS4, WAGR, WIT-2, WT33 WT1 transcription factor Wilms tumor protein|Wilms tumor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WT1, check out the WT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Wilms tumor protein WT1 Antibody (A00199)
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