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Product Info Summary
| SKU: | A09785 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-VRK3 Antibody
SKU/Catalog Number
A09785
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-VRK3 Antibody (Catalog # A09785). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VRK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09785)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human VRK3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09785 is reactive to VRK3 in Human
Reconstitution
Calculated molecular weight
52881 MW
Background of VRK3
Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus By similarity.
Nezu J., Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
Ni X., Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09785 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO205 cells, using VRK3 antibody A09785.
Protein Target Info & Infographic
Gene/Protein Information For VRK3 (Source: Uniprot.org, NCBI)
Gene Name
VRK3
Full Name
Inactive serine/threonine-protein kinase VRK3
Weight
52881 MW
Superfamily
protein kinase superfamily
Alternative Names
Inactive serine/threonine-protein kinase VRK3;Serine/threonine-protein pseudokinase VRK3;Vaccinia-related kinase 3;VRK3; VRK3 VRK serine/threonine kinase 3 inactive serine/threonine-protein kinase VRK3|serine/threonine-protein kinase VRK3|serine/threonine-protein pseudokinase VRK3|vaccinia related kinase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VRK3, check out the VRK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VRK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VRK3 Antibody (A09785)
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No publications found for A09785
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-VRK3 Antibody
Question
My question regarding product A09785, anti-VRK3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09785 anti-VRK3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-01
Question
Will anti-VRK3 antibody A09785 work on goat WB with right testis?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
Our lab technicians have not validated anti-VRK3 antibody A09785 on goat. You can run a BLAST between goat and the immunogen sequence of anti-VRK3 antibody A09785 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat right testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-25
Question
Is a blocking peptide available for product anti-VRK3 antibody (A09785)?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We do provide the blocking peptide for product anti-VRK3 antibody (A09785). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right testis using anti-VRK3 antibody A09785. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-08
Question
Is this A09785 anti-VRK3 antibody reactive to the isotypes of VRK3?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
The immunogen of A09785 anti-VRK3 antibody is Synthesized peptide derived from Internal of human VRK3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-02
Question
We are currently using anti-VRK3 antibody A09785 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
E. Roberts
Verified customer
Asked: 2015-04-02
Answer
The anti-VRK3 antibody (A09785) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-02
