Anti-Proprotein convertase PC4/PCSK4 Antibody

Product Name

Anti-Proprotein convertase PC4/PCSK4 Antibody

View all Proprotein convertase PC4 Antibodies

SKU/Catalog Number

PA2086

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Proprotein convertase PC4/PCSK4 Antibody catalog # PA2086. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Proprotein convertase PC4/PCSK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2086)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of mouse Proprotein convertase PC4, different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2086 is reactive to Pcsk4 in Mouse, Rat

Applications

PA2086 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

83 kDa

Calculated molecular weight

73214 MW

Background of Proprotein convertase PC4

PCSK4 (Proprotein Convertase, Subtilisin/Kexin-Type, 4), is an enzyme that in humans is encoded by the PCSK4 gene. The gene for PC4 (Pcsk4 locus) was mapped to mouse chromosome 10, close to the adipsin locus and near the gene encoding anti-mullerian hormone. Qiu et al. (2005) found that aberrant processing of pro-IGF2 by PC4 may be a cause of IUGR. PC4 cleaved pro-IGF2 to generate the intermediate processed form, IGF2 (1-102). Mature IGF2 (1-67), likely resulting from further processing by carboxypeptidases, is capable of activating invasive trophoblast cells through AKT phosphorylation. Inhibition of PC4 by a PC4-specific inhibitor blocked pro-IGF2 processing and reduced trophoblast cell migration.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PCSK4 using anti-PCSK4 antibody (PA2086).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody (Catalog # PA2086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD.

Gene/Protein Information For Pcsk4 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp434B217; EC 3.4.21; EC 3.4.21.75; EC 3.4.21.93; MGC34749; PC4EC 3.4.21.-; Proprotein convertase 4; proprotein convertase subtilisin/kexin type 4; SPC5 Pcsk4|AI647044, L21221, NEC 3, NEC3, PC, PC4, SP, SPC5|proprotein convertase subtilisin/kexin type 4|proprotein convertase subtilisin/kexin type 4|KEX2-like endoprotease 3|neuroendocrine convertase 3|prohormone convertase 3

For more info on Pcsk4, check out the Pcsk4 Infographic

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In this infographic, you will see the following information for Pcsk4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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