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Product Info Summary
| SKU: | PB9456 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Vitamin D Receptor/VDR Antibody Picoband®
View all VDR/NR1I1/Vitamin D Receptor Antibodies
SKU/Catalog Number
PB9456
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vitamin D Receptor/VDR Antibody Picoband® catalog # PB9456. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vitamin D Receptor/VDR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9456)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human VDR, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9456 is reactive to VDR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
48289 MW
Background of VDR/NR1I1/Vitamin D Receptor
VDR (Vitamin D Receptor), also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al. (1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al. (2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB (TFIIB). A natural polymorphic variant of VDR, termed F/M4 (missing a FokI restriction site), which lacks only the first 3 amino acids (including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length (f/M1) receptor. Shah et al. (2006) stated that the signaling and oncogenic activity of beta-catenin (CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25- dihydroxyvitamin D3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9456 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Human Placenta Tissue, Rat Kidney Tissue, Rat Liver Tissue, Rat Pancreas Tissue, HELA Whole Cell
IHC: mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of VDR using anti-VDR antibody (PB9456).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Human Placenta Tissue Lysate at 50ug,
Lane 2: Rat Kidney Tissue Lysate at 50ug,
Lane 3: Rat Liver Tissue Lysate at 50ug,
Lane 4: Rat Pancreas Tissue Lysate at 50ug,
Lane 5: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDR antigen affinity purified polyclonal antibody (Catalog # PB9456) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDR at approximately 48 kDa. The expected band size for VDR is at 48 kDa.
Click image to see more details
Figure 2. IHC analysis of VDR using anti-VDR antibody (PB9456).
VDR was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDR Antibody (PB9456) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of VDR using anti-VDR antibody (PB9456).
VDR was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDR Antibody (PB9456) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of VDR using anti-VDR antibody (PB9456).
VDR was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDR Antibody (PB9456) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For VDR (Source: Uniprot.org, NCBI)
Gene Name
VDR
Full Name
Vitamin D3 receptor
Weight
48289 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Vitamin D3 receptor;VDR;1,25-dihydroxyvitamin D3 receptor;Nuclear receptor subfamily 1 group I member 1;VDR;NR1I1; VDR NR1I1, PPP1R163 vitamin D receptor vitamin D3 receptor|1,25-dihydroxyvitamin D3 receptor|nuclear receptor subfamily 1 group I member 1|protein phosphatase 1, regulatory subunit 163|vitamin D (1,25- dihydroxyvitamin D3) receptor|vitamin D nuclear receptor variant 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VDR, check out the VDR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VDR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vitamin D Receptor/VDR Antibody Picoband® (PB9456)
Hello CJ!
PB9456 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
Comparative Transcriptome Analysis of Fetal Skin Reveals Key Genes Related to Hair Follicle Morphogenesis in Cashmere Goats
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Customer Q&As
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16 Customer Q&As for Anti-Vitamin D Receptor/VDR Antibody Picoband®
Question
We have been able to see staining in mouse peripheral blood. Do you have any suggestions? Is anti-Vitamin D Receptor/VDR antibody supposed to stain peripheral blood positively?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
Based on literature peripheral blood does express VDR. Based on Uniprot.org, VDR is expressed in tibia, lens epithelium, placenta, peripheral blood, prostate, among other tissues. Regarding which tissues have VDR expression, here are a few articles citing expression in various tissues:
Lens epithelium, Pubmed ID: 9212063
Peripheral blood, Pubmed ID: 1850412, 16252240
Placenta, Pubmed ID: 15489334
Prostate, Pubmed ID: 22323358
Boster Scientific Support
Answered: 2020-04-14
Question
I would like to test anti-Vitamin D Receptor/VDR antibody PB9456 on human peripheral blood for research purposes, then I may be interested in using anti-Vitamin D Receptor/VDR antibody PB9456 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The products we sell, including anti-Vitamin D Receptor/VDR antibody PB9456, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-03
Question
I was wanting to use your anti-Vitamin D Receptor/VDR antibody for IHC for human peripheral blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human peripheral blood identification?
N. Williams
Verified customer
Asked: 2020-03-24
Answer
You can see on the product datasheet, PB9456 anti-Vitamin D Receptor/VDR antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-24
Question
Would PB9456 anti-Vitamin D Receptor/VDR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
It shows on the product datasheet, PB9456 anti-Vitamin D Receptor/VDR antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-09
Question
Is there a BSA free version of anti-Vitamin D Receptor/VDR antibody PB9456 available?
C. Mitchell
Verified customer
Asked: 2019-09-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vitamin D Receptor/VDR antibody PB9456 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-13
Question
Here is the WB image, lot number and protocol we used for peripheral blood using anti-Vitamin D Receptor/VDR antibody PB9456. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-22
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-Vitamin D Receptor/VDR antibody PB9456. Let me know if you need anything else.
A. Moore
Verified customer
Asked: 2019-07-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
Is a blocking peptide available for product anti-Vitamin D Receptor/VDR antibody (PB9456)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We do provide the blocking peptide for product anti-Vitamin D Receptor/VDR antibody (PB9456). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-22
Question
Can you help my question with product PB9456, anti-Vitamin D Receptor/VDR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9456 anti-Vitamin D Receptor/VDR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-05
Question
We bought anti-Vitamin D Receptor/VDR antibody for IHC on prostate in a previous experiment. I am using mouse, and We intend to use the antibody for WB next. I am interested in examining prostate as well as tibia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-09-13
Answer
I viewed the website and datasheets of our anti-Vitamin D Receptor/VDR antibody and it seems that PB9456 has been validated on mouse in both IHC and WB. Thus PB9456 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-13
Question
Our team were happy with the WB result of your anti-Vitamin D Receptor/VDR antibody. However we have been able to see positive staining in prostate nucleus using this antibody. Is that expected? Could you tell me where is VDR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
According to literature, prostate does express VDR. Generally VDR expresses in nucleus. Regarding which tissues have VDR expression, here are a few articles citing expression in various tissues:
Lens epithelium, Pubmed ID: 9212063
Peripheral blood, Pubmed ID: 1850412, 16252240
Placenta, Pubmed ID: 15489334
Prostate, Pubmed ID: 22323358
Boster Scientific Support
Answered: 2017-11-29
Question
We are currently using anti-Vitamin D Receptor/VDR antibody PB9456 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The anti-Vitamin D Receptor/VDR antibody (PB9456) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-28
Question
I see that the anti-Vitamin D Receptor/VDR antibody PB9456 works with IHC, what is the protocol used to produce the result images on the product page?
N. Carter
Verified customer
Asked: 2017-02-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-02-01
Question
I was wanting to use using your anti-Vitamin D Receptor/VDR antibody for dna-templated studies. Has this antibody been tested with western blotting on intestinal cancer tissue? We would like to see some validation images before ordering.
P. Dhar
Verified customer
Asked: 2016-11-15
Answer
We appreciate your inquiry. This PB9456 anti-Vitamin D Receptor/VDR antibody is tested on human placenta tissue, tissue lysate, rat pancreas tissue, kidney tissue, liver tissue, hela whole cell lysate, mouse intestine tissue, intestinal cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-15
Question
Is this PB9456 anti-Vitamin D Receptor/VDR antibody reactive to the isotypes of VDR?
B. Huang
Verified customer
Asked: 2014-11-18
Answer
The immunogen of PB9456 anti-Vitamin D Receptor/VDR antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human VDR (377-402aa HLLYAKMIQKLADLRSLNEEHSKQYR), different from the related mouse and rat sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-11-18
Question
Would anti-Vitamin D Receptor/VDR antibody PB9456 work for IHC with peripheral blood?
J. Carter
Verified customer
Asked: 2013-06-26
Answer
According to the expression profile of peripheral blood, VDR is highly expressed in peripheral blood. So, it is likely that anti-Vitamin D Receptor/VDR antibody PB9456 will work for IHC with peripheral blood.
Boster Scientific Support
Answered: 2013-06-26
