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Product Info Summary
| SKU: | PA1983 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Vitamin D Receptor/VDR Antibody Picoband®
View all VDR/NR1I1/Vitamin D Receptor Antibodies
SKU/Catalog Number
PA1983
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vitamin D Receptor/VDR Antibody catalog # PA1983. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vitamin D Receptor/VDR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1983)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human VDR, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1983 is reactive to VDR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
450 kDa
Calculated molecular weight
48289 MW
Background of VDR/NR1I1/Vitamin D Receptor
VDR (Vitamin D Receptor), also known as Vitamin D Hormone Receptor, is a member of the nuclear receptor family of transcription factors. Labuda et al. (1991) assigned the VDR gene to 12q12-q14 by in situ hybridization. Using mutation analysis, Jurutka et al. (2000) characterized arg18/arg22, VDR residues immediately N-terminal of the first DNA-binding zinc finger, as vital for contact with the general transcription factor IIB (TFIIB). A natural polymorphic variant of VDR, termed F/M4 (missing a FokI restriction site), which lacks only the first 3 amino acids (including glu2), interacted more efficiently with TFIIB and also possessed elevated transcriptional activity compared with the full-length (f/M1) receptor. Shah et al. (2006) stated that the signaling and oncogenic activity of beta-catenin (CTNNB1) can be repressed by activation of VDR. Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25-dihydroxyvitamin D3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1983 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: MCF-7 Cell, HELA Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-VDR antibody, PA1983, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For VDR (Source: Uniprot.org, NCBI)
Gene Name
VDR
Full Name
Vitamin D3 receptor
Weight
48289 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Vitamin D3 receptor;VDR;1,25-dihydroxyvitamin D3 receptor;Nuclear receptor subfamily 1 group I member 1;VDR;NR1I1; VDR NR1I1, PPP1R163 vitamin D receptor vitamin D3 receptor|1,25-dihydroxyvitamin D3 receptor|nuclear receptor subfamily 1 group I member 1|protein phosphatase 1, regulatory subunit 163|vitamin D (1,25- dihydroxyvitamin D3) receptor|vitamin D nuclear receptor variant 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VDR, check out the VDR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VDR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vitamin D Receptor/VDR Antibody Picoband® (PA1983)
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Customer Q&As
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16 Customer Q&As for Anti-Vitamin D Receptor/VDR Antibody Picoband®
Question
I was wanting to use your anti-Vitamin D Receptor/VDR antibody for WB for rat tibia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat tibia identification?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
As indicated on the product datasheet, PA1983 anti-Vitamin D Receptor/VDR antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-28
Question
Is this PA1983 anti-Vitamin D Receptor/VDR antibody reactive to the isotypes of VDR?
M. Wu
Verified customer
Asked: 2019-12-10
Answer
The immunogen of PA1983 anti-Vitamin D Receptor/VDR antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human VDR (389-404aa DLRSLNEEHSKQYRCL), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-10
Question
See attached the WB image, lot number and protocol we used for tibia using anti-Vitamin D Receptor/VDR antibody PA1983. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
I see that the anti-Vitamin D Receptor/VDR antibody PA1983 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-28
Question
My question regarding product PA1983, anti-Vitamin D Receptor/VDR antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1983 anti-Vitamin D Receptor/VDR antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-18
Question
Is there a BSA free version of anti-Vitamin D Receptor/VDR antibody PA1983 available?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vitamin D Receptor/VDR antibody PA1983 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-04
Question
My colleagues were satisfied with the WB result of your anti-Vitamin D Receptor/VDR antibody. However we have been able to see positive staining in tibia nucleus using this antibody. Is that expected? Could you tell me where is VDR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
From what I have seen in literature, tibia does express VDR. Generally VDR expresses in nucleus. Regarding which tissues have VDR expression, here are a few articles citing expression in various tissues:
Lens epithelium, Pubmed ID: 9212063
Peripheral blood, Pubmed ID: 1850412, 16252240
Placenta, Pubmed ID: 15489334
Prostate, Pubmed ID: 22323358
Boster Scientific Support
Answered: 2019-08-14
Question
We have observed staining in human peripheral blood. Any tips? Is anti-Vitamin D Receptor/VDR antibody supposed to stain peripheral blood positively?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
From literature peripheral blood does express VDR. From Uniprot.org, VDR is expressed in tibia, lens epithelium, placenta, peripheral blood, prostate, among other tissues. Regarding which tissues have VDR expression, here are a few articles citing expression in various tissues:
Lens epithelium, Pubmed ID: 9212063
Peripheral blood, Pubmed ID: 1850412, 16252240
Placenta, Pubmed ID: 15489334
Prostate, Pubmed ID: 22323358
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use using your anti-Vitamin D Receptor/VDR antibody for rickets vitamin d-dependent 2a (vddr2a) studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
L. Lewis
Verified customer
Asked: 2019-07-22
Answer
Thanks for your inquiry. This PA1983 anti-Vitamin D Receptor/VDR antibody is tested on hela cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-Vitamin D Receptor/VDR antibody PA1983. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-01
Question
We are interested in to test anti-Vitamin D Receptor/VDR antibody PA1983 on rat tibia for research purposes, then I may be interested in using anti-Vitamin D Receptor/VDR antibody PA1983 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Roberts
Verified customer
Asked: 2017-05-04
Answer
The products we sell, including anti-Vitamin D Receptor/VDR antibody PA1983, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-04
Question
Is a blocking peptide available for product anti-Vitamin D Receptor/VDR antibody (PA1983)?
G. Li
Verified customer
Asked: 2016-11-17
Answer
We do provide the blocking peptide for product anti-Vitamin D Receptor/VDR antibody (PA1983). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-11-17
Question
Would PA1983 anti-Vitamin D Receptor/VDR antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Taylor
Verified customer
Asked: 2016-10-12
Answer
As indicated on the product datasheet, PA1983 anti-Vitamin D Receptor/VDR antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-10-12
Question
Does anti-Vitamin D Receptor/VDR antibody PA1983 work for WB with tibia?
R. Bhatt
Verified customer
Asked: 2016-07-25
Answer
According to the expression profile of tibia, VDR is highly expressed in tibia. So, it is likely that anti-Vitamin D Receptor/VDR antibody PA1983 will work for WB with tibia.
Boster Scientific Support
Answered: 2016-07-25
Question
We are currently using anti-Vitamin D Receptor/VDR antibody PA1983 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
F. Edwards
Verified customer
Asked: 2016-03-28
Answer
The anti-Vitamin D Receptor/VDR antibody (PA1983) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-28
Question
Does anti-Vitamin D Receptor/VDR antibody PA1983 work on primate WB with prostate?
B. Taylor
Verified customer
Asked: 2013-08-29
Answer
Our lab technicians have not validated anti-Vitamin D Receptor/VDR antibody PA1983 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Vitamin D Receptor/VDR antibody PA1983 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate prostate in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-29
