Anti-VAPA Antibody Picoband®

VAP-A antibody

Boster Bio Anti-VAPA Antibody Picoband® catalog # A05329-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A05329-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-VAPA Antibody Picoband®

View all VAP-A Antibodies

SKU/Catalog Number

A05329-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VAPA Antibody Picoband® catalog # A05329-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-VAPA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05329-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human VAPA, which shares 94.1% amino acid (aa) sequence identity with mouse and rat VAPA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05329-1 is reactive to VAPA in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

33 kDa

Calculated molecular weight

39411 MW

Background of VAP-A

VAMP-Associated Protein A ( or Vesicle-Associated Membrane Protein-Associated Protein A) is a protein that in humans is encoded by the VAPA gene. The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05329-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human

Positive Control

WB: human Hela whole cell, human K562 whole cell, human 293T whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue
IHC: human cervical cancer tissue, human colorectal adenocarcinoma tissue, human lung adenocarcinoma tissue, human placenta tissue, mouse colon tissue, rat colon tissue
ICC/IF: PC-3 cell
IF: human colorectal adenocarcinoma tissue, rat colon tissue
FCM: U87 cell

Validation Images & Assay Conditions

Gene/Protein Information For VAPA (Source: Uniprot.org, NCBI)

Gene Name

VAPA

Full Name

Vesicle-associated membrane protein-associated protein A

Weight

39411 MW

Superfamily

VAMP-associated protein (VAP) (TC 9.B.17) family

Alternative Names

Cytoskeleton-associated protein 5; Colonic and hepatic tumor overexpressed gene protein; Ch-TOG; CKAP5; KIAA0097 VAPA VAMP-A, VAP-33, VAP-A, VAP33, hVAP-33 VAMP associated protein A vesicle-associated membrane protein-associated protein A|33 kDa VAMP-associated protein|VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VAPA, check out the VAPA Infographic

VAPA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VAPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A05329-1

100μg

$370
A05329-1-10ug

10μg sample (liquid)

$99
A05329-1-Biotin

100 μg Biotin conjugated

$570
A05329-1-Cy3

100 μg Cy3 conjugated

$570
A05329-1-Dylight488

100 μg Dylight488 conjugated

$570
A05329-1-Dylight550

100 μg Dylight550 conjugated

$570
A05329-1-Dylight594

100 μg Dylight594 conjugated

$570
A05329-1-FITC

100 μg FITC conjugated

$570
A05329-1-HRP

100 μg HRP conjugated

$570
A05329-1-APC

100 μg APC conjugated

$670
A05329-1-PE

100 μg PE conjugated

$670
A05329-1-iFluor647

100 μg iFluor647 conjugated

$670
A05329-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A05329-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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