Anti-Uteroglobin/Scgb1a1 Antibody Picoband™

Product Name

Anti-Uteroglobin/Scgb1a1 Antibody Picoband™

View all Uteroglobin/SCGB1A1 Antibodies

SKU/Catalog Number

A02201-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Uteroglobin/Scgb1a1 Antibody Picoband™ catalog # A02201-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Uteroglobin/Scgb1a1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02201-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat Uteroglobin recombinant protein (Position: S20-V96).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02201-2 is reactive to SCGB1A1 in Rat

Applications

A02201-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

10 kDa

Calculated molecular weight

31568 MW

Background of Uteroglobin/SCGB1A1

Uteroglobin, also known as secretoglobin family 1A member 1 (SCGB1A1), is a protein that in humans is encoded by the SCGB1A1 gene. This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA(Cap), 1-5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Uteroglobin using anti-Uteroglobin antibody (A02201-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysate,
Lane 2: rat lung tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uteroglobin antigen affinity purified polyclonal antibody (Catalog # A02201-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uteroglobin at approximately 10KD. The expected band size for Uteroglobin is at 10KD.

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Figure 2. IHC analysis of Uteroglobin using anti-Uteroglobin antibody (A02201-2). Uteroglobin was detected in paraffin-embedded section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Uteroglobin Antibody (A02201-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. Sandwich ELISA - Recombinant rat SCGB1A1 protein standard curve.
Use in combination with reagents from Rat SCGB1A1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1636).

Gene/Protein Information For SCGB1A1 (Source: Uniprot.org, NCBI)

Alternative Names

Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin SCGB1A1 CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1 secretoglobin family 1A member 1 uteroglobin|blastokinin|clara cell phospholipid-binding protein|clara cells 10 kDa secretory protein|secretoglobin, family 1A, member 1 (uteroglobin)|urinary protein 1|urine protein 1

For more info on SCGB1A1, check out the SCGB1A1 Infographic

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In this infographic, you will see the following information for SCGB1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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