Click image to see more details
Product Info Summary
| SKU: | EK1636 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Rat SCGB1A1/uteroglobin ELISA Kit PicoKine®
View all Uteroglobin/SCGB1A1 ELISA kits
SKU/Catalog Number
EK1636
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat SCGB1A1/uteroglobin ELISA Kit PicoKine® (96 Tests). Quantitate Rat Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SCGB1A1/uteroglobin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1636)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S20-V96
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1636 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1636 is reactive to Scgb1a1 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK1636 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Uteroglobin/SCGB1A1
SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1636-CAP | Anti-Rat Scgb1a1 Pre-coated 96-well strip microplate | 1 |
| EK1636-ST | Rat Scgb1a1 Standard | 2 vials, 10 ng/tube |
| EK1636-DA | Rat Scgb1a1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10000 |
| O.D. | 0.042 | 0.075 | 0.099 | 0.154 | 0.263 | 0.522 | 1.135 | 2.156 |
Data Example Images
Click image to see more details
Rat SCGB1A1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Uteroglobin/SCGB1A1, Dilution ratio of 1:10, concentration in serum and plasma is around 20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SCGB1A1/uteroglobin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 220 | 1610 | 4490 | 241 | 1635 | 4849 |
| Standard deviation | 8.8 | 123.97 | 298.6%4 | 10.36 | 140.61 | 374.3%2 |
| CV (%) | 4% | 7.7% | 6.6% | 4.3% | 8.6% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Uteroglobin/SCGB1A1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 220 | 258 | 259 | 253 | 247 | 16.03 | 6.4% |
| Sample 2 | 1610 | 1483 | 1380 | 1552 | 1506 | 85.63 | 5.6% |
| Sample 3 | 4490 | 4875 | 4487 | 4849 | 4675 | 186.97 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For Scgb1a1 (Source: Uniprot.Org, NCBI)
Gene Name
Scgb1a1
Full Name
Uteroglobin
Weight
9.994kDa
Superfamily
secretoglobin family
Alternative Names
Uteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; PCB-binding protein; Secretoglobin family 1A member 1; Scgb1a1; Cc10; Ugb; Utg SCGB1A1 CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1 secretoglobin family 1A member 1 uteroglobin|blastokinin|clara cell phospholipid-binding protein|clara cells 10 kDa secretory protein|secretoglobin, family 1A, member 1 (uteroglobin)|urinary protein 1|urine protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Scgb1a1, check out the Scgb1a1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Scgb1a1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat SCGB1A1/uteroglobin ELISA Kit PicoKine® (EK1636)
Hello CJ!
No publications found for EK1636
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Rat SCGB1A1/uteroglobin ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Rat SCGB1A1/uteroglobin ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Rat SCGB1A1/uteroglobin ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-12
Question
Q: how to analyze ELISA data? I have performed Uteroglobin level in serum.
C. Kulkarni
Verified customer
Asked: 2019-09-16
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-09-16
Question
Q: if the enzyme conjugated Uteroglobin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Uteroglobin antigen?
R. Campbell
Verified customer
Asked: 2018-04-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-04-19
Question
Q: how can I thaw whole blood sample for Uteroglobin ELISA after freezing?
D. Garcia
Verified customer
Asked: 2016-10-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Uteroglobin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-10-20
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
D. Jackson
Verified customer
Asked: 2016-10-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-10-15
Question
Q: can I use citrate plasma as samples in Rat Uteroglobin Picokine® ELISA Kit (Catalog # EK1636)?
Verified Customer
Verified customer
Asked: 2016-09-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Uteroglobin causing disruption of its protein structure. Uteroglobin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Uteroglobin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-14
