Anti-USP25 Antibody

Product Name

Anti-USP25 Antibody

View all USP25 Antibodies

SKU/Catalog Number

M06182

Size

80 µl

Description

Boster Bio Anti-USP25 Antibody (Catalog # M06182). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-USP25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06182)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1277CT376.106.171

Isotype

IgG1,κ

Immunogen

This USP25 antibody is generated from a mouse immunized with a recombinant protein from human USP25.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06182 is reactive to USP25 in Human

Applications

M06182 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

122218 Da

Background of USP25

Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot analysis of lysate from Daudi cell line, using USP25 Antibody. M06182 was diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35μg.

Gene/Protein Information For USP25 (Source: Uniprot.org, NCBI)

Alternative Names

Deubiquitinating enzyme 25; EC 3.1.2.15; EC 3.4.19.12; ubiquitin carboxyl-terminal hydrolase 25; ubiquitin specific peptidase 25; ubiquitin specific protease USP2510USP21ubiquitin specific protease 25; ubiquitin thioesterase 25; Ubiquitin Thiolesterase 25; ubiquitin-specific processing protease 25; Ubiquitin-specific-processing protease 25; USP on chromosome 21; USP25 USP25 USP21 ubiquitin specific peptidase 25 ubiquitin carboxyl-terminal hydrolase 25|USP on chromosome 21|deubiquitinating enzyme 25|ubiquitin thioesterase 25|ubiquitin thiolesterase 25|ubiquitin-specific processing protease 25

For more info on USP25, check out the USP25 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for USP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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