Anti-GEMC1 GMNC Antibody

Product Name

Anti-GEMC1 GMNC Antibody

View all GEMC1 Antibodies

SKU/Catalog Number

A17100

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GEMC1 GMNC Antibody (Catalog # A17100). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

GEMC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GEMC1 GMNC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17100)

Host

Rabbit

Contents

GEMC1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GEMC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human GEMC1. The immunogen is located within amino acids 130 - 180 of GEMC1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

OGFOD2 antibody is predicted to not cross-react with other TPA1 protein family members. At least three isoforms are known to exist; this antibody will only detect the two larger isoforms.

Reactive Species

A17100 is reactive to GEMC1 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

37887 MW

Background of GEMC1

The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A17100 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GEMC1 antibody can be used for detection of GEMC1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For GEMC1 (Source: Uniprot.org, NCBI)

Alternative Names

GEMC1, GEMC1, Geminin coiled-coil domain-containing protein 1 GMNC GEMC1 geminin coiled-coil domain containing geminin coiled-coil domain-containing protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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