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Product Info Summary
| SKU: | A06182 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-USP25 Antibody
SKU/Catalog Number
A06182
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-USP25 Antibody (Catalog # A06182). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
USP25 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-USP25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06182)
Host
Rabbit
Contents
USP25 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25. The immunogen is located within amino acids 470 - 520 of USP25.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.
Reactive Species
A06182 is reactive to USP25 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
122218 MW
Background of USP25
USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06182 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 μg/mL.
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Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For USP25 (Source: Uniprot.org, NCBI)
Gene Name
USP25
Full Name
Ubiquitin carboxyl-terminal hydrolase 25
Weight
122218 MW
Superfamily
peptidase C19 family
Alternative Names
USP21, USP21, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25 USP25 USP21 ubiquitin specific peptidase 25 ubiquitin carboxyl-terminal hydrolase 25|USP on chromosome 21|deubiquitinating enzyme 25|ubiquitin thioesterase 25|ubiquitin thiolesterase 25|ubiquitin-specific processing protease 25
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP25, check out the USP25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP25 Antibody (A06182)
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