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Product Info Summary
| SKU: | A00993-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-uPA Receptor/U-PAR/PLAUR Antibody Picoband®
SKU/Catalog Number
A00993-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-uPA Receptor/U-PAR/PLAUR Antibody Picoband® catalog # A00993-4. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-uPA Receptor/U-PAR/PLAUR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00993-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human uPA Receptor/U-PAR/PLAUR recombinant protein (Position: E128-R167).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00993-4 is reactive to PLAUR in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
36.978kDa
Background of uPAR
PLAUR(PLASMINOGEN ACTIVATOR RECEPTOR, UROKINASE-TYPE), also known as UPAR or CD87, is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. PLAUR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. PLAUR is originally identified as a saturable binding site for urokinase on the cell surface. And the gene plays an important role in many normal as well as pathologic processes. The PLAUR gene is localized to 19q13.31. PLAUR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. PLAUR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus it seems to be an important player in the regulation of this process. In human coronary artery vascular smooth muscle cells, UPA stimulates cell migration via a UPAR signaling complex containing TYK2 and phosphatidylinositol 3-kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00993-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human Raji whole cell, human COLO-320 whole cell, human MCF-7 whole cell, rat brain tissue, rat thymus tissue, mouse brain tissue,
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of UPA Receptor/U-PAR/PLAUR using anti-UPA Receptor/U-PAR/PLAUR antibody (A00993-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse brain tissue lysates,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPA Receptor/U-PAR/PLAUR antigen affinity purified polyclonal antibody (Catalog # A00993-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPA Receptor/U-PAR/PLAUR at approximately 45 kDa. The expected band size for UPA Receptor/U-PAR/PLAUR is at 45 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PLAUR (Source: Uniprot.org, NCBI)
Gene Name
PLAUR
Full Name
Urokinase plasminogen activator surface receptor
Weight
36.978kDa
Alternative Names
Urokinase plasminogen activator surface receptor; U-PAR; Monocyte activation antigen Mo3; uPAR; CD87; Plaur; MO3; UPAR PLAUR CD87, U-PAR, UPAR, URKR plasminogen activator, urokinase receptor urokinase plasminogen activator surface receptor|monocyte activation Mo3|u-plasminogen activator receptor form 2|urokinase-type plasminogen activator (uPA) receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLAUR, check out the PLAUR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLAUR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-uPA Receptor/U-PAR/PLAUR Antibody Picoband® (A00993-4)
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