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Product Info Summary
| SKU: | M07706 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ULK3 Monoclonal Antibody
SKU/Catalog Number
M07706
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ULK3 Monoclonal Antibody catalog # M07706. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ULK3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07706)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACHC-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ULK3 Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07706 is reactive to ULK3 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
35575 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07706 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of ULK3 expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ULK3 (Source: Uniprot.org, NCBI)
Gene Name
ULK3
Full Name
Serine/threonine-protein kinase ULK3
Weight
35575 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform;PP2A-beta;3.1.3.16;PPP2CB; ULK3 unc-51 like kinase 3 serine/threonine-protein kinase ULK3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ULK3, check out the ULK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ULK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ULK3 Monoclonal Antibody (M07706)
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No publications found for M07706
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Customer Q&As
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3 Customer Q&As for Anti-ULK3 Monoclonal Antibody
Question
Would M07706 anti-ULK3 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Anderson
Verified customer
Asked: 2019-12-20
Answer
It shows on the product datasheet, M07706 anti-ULK3 Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-20
Question
Is a blocking peptide available for product anti-ULK3 Monoclonal antibody (M07706)?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
We do provide the blocking peptide for product anti-ULK3 Monoclonal antibody (M07706). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-03-06
Question
We are interested in to test anti-ULK3 Monoclonal antibody M07706 on mouse testis for research purposes, then I may be interested in using anti-ULK3 Monoclonal antibody M07706 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
The products we sell, including anti-ULK3 Monoclonal antibody M07706, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-26
