Anti-ULK3 Antibody

Product Name

Anti-ULK3 Antibody

View all ULK3 Antibodies

SKU/Catalog Number

A07706

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-ULK3 Antibody (Catalog # A07706). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

ULK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-ULK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07706)

Host

Rabbit

Contents

ULK3 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3. The immunogen is located within amino acids 230 - 280 of ULK3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Reactive Species

A07706 is reactive to ULK3 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

53444 MW

Background of ULK3

ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07706 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For ULK3 (Source: Uniprot.org, NCBI)

Alternative Names

Unc-51-like kinase 3 ULK3 unc-51 like kinase 3 serine/threonine-protein kinase ULK3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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