Anti-UIMC1 Antibody (C-term)

Product Name

Anti-UIMC1 Antibody (C-term)

View all RAP80 Antibodies

SKU/Catalog Number

A06262-2

Size

80 µl

Description

Boster Bio Anti-UIMC1 Antibody (C-term) (Catalog # A06262-2). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-UIMC1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06262-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This UIMC1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 567-601 amino acids from the C-terminal region of human UIMC1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06262-2 is reactive to Uimc1 in Human

Applications

A06262-2 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

79727 Da

Background of RAP80

Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
FC: 1:25

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of lysate from HT-1080 cell line, using UIMC1 Antibody (C-term). A06262-2 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Click image to see more details

Flow cytometric analysis of MCF-7 cells using UIMC1 Antibody (C-term)(green, ) compared to an isotype control of rabbit IgG(blue). A06262-2 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Gene/Protein Information For Uimc1 (Source: Uniprot.org, NCBI)

Alternative Names

BRCA1-A complex subunit RAP80; RAP80; RAP80X2HRIP110; receptor associated protein 80; Receptor-associated protein 80; retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RXRIP110; ubiquitin interaction motif containing 1; Ubiquitin interaction motif-containing protein 1; UIMC1; X2HRIP110 Uimc1|9430016E08Rik, D330018D10Rik, D630032M02Rik, RIP110, Rxri, Rxrip110|ubiquitin interaction motif containing 1|BRCA1-A complex subunit RAP80|receptor-associated protein 80|retinoid X receptor interacting protein 110|ubiquitin interaction motif-containing protein 1

For more info on Uimc1, check out the Uimc1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Uimc1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Uimc1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis