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Product Info Summary
| SKU: | A00350 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Leptin Receptor/LEPR Antibody
SKU/Catalog Number
A00350
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Leptin Receptor/LEPR Antibody catalog # A00350. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Leptin Receptor/LEPR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00350)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Leptin Receptor, different from the related mouse sequence by nine amino acids, and from the related rat sequence by eight amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00350 is reactive to LEPR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
27 kDa
Calculated molecular weight
132494 MW
Background of Leptin R
Leptin receptor, also known as LEP-R or OB-R is a protein that in humans is encoded by the LEPR gene. The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00350 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: mouse kidney tissue, rat lung tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350).
Leptin Receptor/LEPR was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Leptin Receptor/LEPR Antibody (A00350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350).
Leptin Receptor/LEPR was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Leptin Receptor/LEPR Antibody (A00350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For LEPR (Source: Uniprot.org, NCBI)
Gene Name
LEPR
Full Name
Leptin receptor
Weight
132494 MW
Superfamily
type I cytokine receptor family
Alternative Names
Leptin receptor;LEP-R;HuB219;OB receptor;OB-R;CD295;LEPR;DB, OBR; LEPR CD295, LEP-RD, OB-R, OBR, LEPR leptin receptor leptin receptor|OB receptor|huB219
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LEPR, check out the LEPR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LEPR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Leptin Receptor/LEPR Antibody (A00350)
Hello CJ!
A00350 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Immunohistochemical Localization of Leptin and Leptin-Receptor Proteins in Different Tissues of Chinese Alligator, Alligator sinensis. HuaBin Zhang,YaSen Li,Tao Pan,Peng Yan,En Li,Hui Xue,XiaoBing Wu
Species: Chinese Alligator
A00350 usage in article: APP:IHC, SAMPLE:TESTIS,SUPERMADUCT,EPIDIDYMIS,OVARY,OVIDUCT,STOMACH,INTESTINE AND ADIPOSE TISSUE, DILUTION:1:100
The Influence of LepR Tyrosine Site Mutations on Mouse Ovary Development and Related Gene Expression Changes
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1 Customer Q&As for Anti-Leptin Receptor/LEPR Antibody
Question
We are currently using anti-Leptin Receptor/LEPR antibody A00350 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Leptin Receptor/LEPR antibody (A00350) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
