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Product Info Summary
| SKU: | A00255 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-UCP1 Antibody
SKU/Catalog Number
A00255
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-UCP1 Antibody (Catalog # A00255). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
UCP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-UCP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00255)
Host
Rabbit
Contents
UCP1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
UCP1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human UCP1 (GenBank accession. The immunogen is located within the last 50 amino acids of UCP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
UCP1 antibody is human, mouse and rat reactive.
Reactive Species
A00255 is reactive to UCP1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
33005 MW
Background of UCP1
The mitochondrial brown fat uncoupling protein 1 (UCP1) is a member of the family of mitochondrial anion carrier proteins (MACP) (1). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells (1). UCP1 is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00255 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
UCP1 antibody can be used for detection of UCP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of UCP1 in human brain tissue lysate with UCP1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of UCP1 in human liver tissue with UCP1 antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For UCP1 (Source: Uniprot.org, NCBI)
Gene Name
UCP1
Full Name
Mitochondrial brown fat uncoupling protein 1
Weight
33005 MW
Superfamily
mitochondrial carrier (TC 2.A.29) family
Alternative Names
UCP, SLC25A7, UCP, Mitochondrial brown fat uncoupling protein 1, Solute carrier family 25 member 7, UCP 1 UCP1 SLC25A7, UCP uncoupling protein 1 mitochondrial brown fat uncoupling protein 1|solute carrier family 25 member 7|thermogenin|uncoupling protein 1 (mitochondrial, proton carrier)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UCP1, check out the UCP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UCP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UCP1 Antibody (A00255)
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