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Product Info Summary
| SKU: | PA2189 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband®
SKU/Catalog Number
PA2189
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Monocarboxylate transporter 5 SLC16A4 Antibody catalog # PA2189. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2189)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC16A4, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2189 is reactive to SLC16A4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
274 kDa
Calculated molecular weight
54022 MW
Background of SLC16A4
Monocarboxylate transporter 5, also called Solute carrier family 16 member 4 or MCT4, is a protein that in humans is encodes by the SLC16A4 gene. This gene is mapped to 1p13.3. It acts as the proton-linked monocarboxylate transporter. This gene catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2189 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis Tissue, JURKAT Cell, HELA Cell, MCF-7 Cell
IHC: Human Mammary Cancer Tissue, Human Placenta Cancer Tissue
Validation Images & Assay Conditions
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Anti-SLC16A4 antibody, PA2189, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
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Anti-SLC16A4 antibody, PA2189, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-SLC16A4 antibody, PA2189, IHC(P)
IHC(P): Human Placenta Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For SLC16A4 (Source: Uniprot.org, NCBI)
Gene Name
SLC16A4
Full Name
Monocarboxylate transporter 5
Weight
54022 MW
Superfamily
major facilitator superfamily
Alternative Names
Monocarboxylate transporter 5;MCT 5;Monocarboxylate transporter 4;MCT 4;Solute carrier family 16 member 4;SLC16A4;MCT4, MCT5; SLC16A4 MCT4, MCT5 solute carrier family 16 member 4 monocarboxylate transporter 5|MCT 4|MCT 5|monocarboxylate transporter 4|solute carrier family 16 (monocarboxylic acid transporters), member 4|solute carrier family 16, member 4 (monocarboxylic acid transporter 5)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC16A4, check out the SLC16A4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC16A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband® (PA2189)
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Customer Q&As
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7 Customer Q&As for Anti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband®
Question
I was wanting to use your anti-SLC16A4 antibody for IHC for mouse palpebral conjunctiva on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse palpebral conjunctiva identification?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
It shows on the product datasheet, PA2189 anti-SLC16A4 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse palpebral conjunctiva in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
Is this PA2189 anti-SLC16A4 antibody reactive to the isotypes of SLC16A4?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The immunogen of PA2189 anti-SLC16A4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SLC16A4(134-148aa VVTTKYFKKRLALST), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-09
Question
Can you help my question with product PA2189, anti-SLC16A4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2189 anti-SLC16A4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-30
Question
I see that the anti-SLC16A4 antibody PA2189 works with IHC, what is the protocol used to produce the result images on the product page?
P. Jackson
Verified customer
Asked: 2018-08-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-31
Question
My lab would like to test anti-SLC16A4 antibody PA2189 on mouse palpebral conjunctiva for research purposes, then I may be interested in using anti-SLC16A4 antibody PA2189 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
The products we sell, including anti-SLC16A4 antibody PA2189, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-22
Question
We are currently using anti-SLC16A4 antibody PA2189 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
L. Walker
Verified customer
Asked: 2016-01-26
Answer
The anti-SLC16A4 antibody (PA2189) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-26
Question
Will anti-SLC16A4 antibody PA2189 work on monkey WB with placenta substantia nigra?
A. Zhang
Verified customer
Asked: 2015-02-09
Answer
Our lab technicians have not validated anti-SLC16A4 antibody PA2189 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-SLC16A4 antibody PA2189 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey placenta substantia nigra in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-09
