Product Info Summary
SKU: | A07315-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC-P, WB |
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Product info
Product Name
Anti-UBE2L6 Antibody (N-term)
View all UbcH8/Ube2L6 Antibodies
SKU/Catalog Number
A07315-1
Size
80 µl
Description
Boster Bio Anti-UBE2L6 Antibody (N-term) (Catalog # A07315-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-UBE2L6 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07315-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This UBE2L6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2L6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07315-1 is reactive to UBE2L6 in Human
Applications
A07315-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
17769 Da
Background of UbcH8/Ube2L6
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L6 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to function in the E6/E6-ap-induced ubiquitination of p53.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysate from HL-60 cell line, using UBE2L6 Antibody (M1). UBE2L6 Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Click image to see more details
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with UBE2L6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For UBE2L6 (Source: Uniprot.org, NCBI)
Gene Name
UBE2L6
Full Name
Ubiquitin/ISG15-conjugating enzyme E2 L6
Weight
17769 Da
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
EC 6.3.2.19; retinoic acid induced gene B protein; Retinoic acid-induced gene B protein; RIG-B; UbcH8; UBCH8MGC40331; UBE2L6; Ubiquitin carrier protein L6; ubiquitin/ISG15-conjugating enzyme E2 L6; ubiquitin-conjugating enzyme E2L 6; Ubiquitin-protein ligase L6 UBE2L6 RIG-B, UBCH8 ubiquitin conjugating enzyme E2 L6 ubiquitin/ISG15-conjugating enzyme E2 L6|E2 ubiquitin-conjugating enzyme L6|retinoic acid induced gene B protein|ubiquitin carrier protein L6|ubiquitin conjugating enzyme E2L 6|ubiquitin-protein ligase L6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2L6, check out the UBE2L6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2L6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE2L6 Antibody (N-term) (A07315-1)
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