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Product Info Summary
| SKU: | A10490 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RNF144b/Ibrdc2 Antibody
SKU/Catalog Number
A10490
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RNF144b/Ibrdc2 Antibody catalog # A10490. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF144b/Ibrdc2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10490)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human p53RFP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10490 is reactive to RNF144B in Human, Mouse
Reconstitution
Calculated molecular weight
33697 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10490 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using p53RFP Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using p53RFP Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For RNF144B (Source: Uniprot.org, NCBI)
Gene Name
RNF144B
Full Name
E3 ubiquitin-protein ligase RNF144B
Weight
33697 MW
Superfamily
RBR family
Alternative Names
E3 ubiquitin-protein ligase RNF144B;6.3.2.-;IBR domain-containing protein 2;RING finger protein 144B;p53-inducible RING finger protein;RNF144B;IBRDC2, P53RFP;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF144B, check out the RNF144B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF144B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF144b/Ibrdc2 Antibody (A10490)
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No publications found for A10490
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Customer Q&As
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7 Customer Q&As for Anti-RNF144b/Ibrdc2 Antibody
Question
Is this A10490 anti-RNF144b/Ibrdc2 antibody reactive to the isotypes of RNF144B?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The immunogen of A10490 anti-RNF144b/Ibrdc2 antibody is Synthesized peptide derived from the Internal region of human p53RFP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-28
Question
My question regards to test anti-RNF144b/Ibrdc2 antibody A10490 on mouse quadriceps femoris for research purposes, then I may be interested in using anti-RNF144b/Ibrdc2 antibody A10490 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The products we sell, including anti-RNF144b/Ibrdc2 antibody A10490, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-31
Question
We are currently using anti-RNF144b/Ibrdc2 antibody A10490 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The anti-RNF144b/Ibrdc2 antibody (A10490) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-01
Question
Would anti-RNF144b/Ibrdc2 antibody A10490 work on bovine WB with prostate testis?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Our lab technicians have not tested anti-RNF144b/Ibrdc2 antibody A10490 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-RNF144b/Ibrdc2 antibody A10490 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine prostate testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-25
Question
I was wanting to use your anti-RNF144b/Ibrdc2 antibody for WB for mouse quadriceps femoris on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse quadriceps femoris identification?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
It shows on the product datasheet, A10490 anti-RNF144b/Ibrdc2 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse quadriceps femoris in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-14
Question
Is a blocking peptide available for product anti-RNF144b/Ibrdc2 antibody (A10490)?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
We do provide the blocking peptide for product anti-RNF144b/Ibrdc2 antibody (A10490). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-03-06
Question
Does A10490 anti-RNF144b/Ibrdc2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Jackson
Verified customer
Asked: 2016-01-07
Answer
It shows on the product datasheet, A10490 anti-RNF144b/Ibrdc2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-01-07
