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- Table of Contents
Facts about Splicing factor U2AF 65 kDa subunit.
Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly.
Human | |
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Gene Name: | U2AF2 |
Uniprot: | P26368 |
Entrez: | 11338 |
Belongs to: |
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splicing factor SR family |
hU2AF(65); hU2AF65; splicing factor U2AF 65 kD subunit; splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear RNA auxiliary factor 2; U2AF65U2 snRNP auxiliary factor large subunit
Mass (kDA):
53.501 kDA
Human | |
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Location: | 19q13.42 |
Sequence: | 19; NC_000019.10 (55654132..55674716) |
Nucleus.
Steven Boster was one the most successful entrepreneurs of 1990s and the inventor of many cutting edge biotechnology products. As an immunohistochemistry (IHC) pioneer, Boster earned the nickname "he who converts science in the lavatory". He invented hundreds of primary antibodies and developed many IHC products. His company became China's largest catalog antibody manufacturer in the late nineties. PicoKine(tm) is Boster's most recent invention. It is a proprietary ELISA platform. This platform produces highly sensitive, specific ELISA kits.
Functional connectivity studies can be notoriously difficult to interpret, due to both technical as well as biological confounds. Biological confounds affect the noise spectrum of data, influencing the likelihood that functional interactions are spurious. However, these studies can provide valuable information regarding biological processes and disease mechanisms. These studies are particularly useful for identifying genes which could be therapeutic. They can also reveal genetic variants' impact on human disease or cancer development.
The Cell Counting Kit-8 can be used to measure cytotoxicity and cell proliferation. It can be added to cell culture media without the need to kill cells, making it an excellent choice for high-throughput screening. Its sensitivity is superior to MTT, WST-1. HeLa cells were incubated for two hours with WST-8 and/or WST-1 to use the Cell Counting Kit-8. Cell Counting Kit-8 was then added to the cells.
The CCK-8 Kit is available in a readymade solution that can keep at 4oC up to one year. The data from this kit show that cell proliferation is not sufficient to detect death. The CK12 kit is superior to CCK-8, as it also measures LDH from dying cells. A microplate reader equipped for measuring LDH concentrations is needed.
It is important to note that the survival rate for NSCLC patients is poor, with a five-year survival rate of only 15%. Understanding the biology of lung cancer is crucial for improving prognosis, and clinical therapy. Researchers can use the Cell Counting Kit-8 to deterluer uonswitof C'iproprieteytotoxicitherapy. Researchers carFnatftatalwellsenin a physt ta neterluer uonswrcheexto deng proprietherapyliniThe CCK-8 Kit iallows physt ta nelinioell a witrocessef an inteneterr tprodutive, speentlslymetrriaanteynd dterluer uonswrcheviabils suteytote laiss bore media withcan bund d and/,h couldproprwf or-ion n a tetrazratum-salt. Wddedof higd,lso ces highlaly sensiabsorbin a tion zseasye. Onswsamplonly Kit ise ttataleasurort then 50,000s. A micell Counting Kit-8 can bwellsed to or iitoproligrowtldprogeraarnethen urvivherapyof only r is crunof C'iaiss bp>
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PMID: 1538748 by Zamore P.D., et al. Cloning and domain structure of the mammalian splicing factor U2AF.
PMID: 12176931 by Rappsilber J., et al. Large-scale proteomic analysis of the human spliceosome.