Click image to see more details
Product Info Summary
| SKU: | M00326-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tyrosinase Rabbit Monoclonal Antibody
View all Tyrosinase Antibodies
SKU/Catalog Number
M00326-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Tyrosinase Rabbit Monoclonal Antibody catalog # M00326-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosinase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00326-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAHE-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tyrosinase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00326-1 is reactive to TYR in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
60393 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00326-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human melanoma
Validation Images & Assay Conditions
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Western blot analysis of Tyrosinase expression in human melanoma lysate.
Protein Target Info & Infographic
Gene/Protein Information For TYR (Source: Uniprot.org, NCBI)
Gene Name
TYR
Full Name
Tyrosinase
Weight
60393 MW
Superfamily
tyrosinase family
Alternative Names
Tyrosinase;1.14.18.1;LB24-AB;Monophenol monooxygenase;SK29-AB;Tumor rejection antigen AB;TYR; TYR ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3 tyrosinase tyrosinase|LB24-AB|SK29-AB|monophenol monooxygenase|oculocutaneous albinism IA|tumor rejection AB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYR, check out the TYR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosinase Rabbit Monoclonal Antibody (M00326-1)
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No publications found for M00326-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Tyrosinase Rabbit Monoclonal Antibody
Question
Would anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 work for WB with melanoma t-cell?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
According to the expression profile of melanoma t-cell, TYR is highly expressed in melanoma t-cell. So, it is likely that anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 will work for WB with melanoma t-cell.
Boster Scientific Support
Answered: 2020-04-01
Question
I would like to test anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 on human melanoma t-cell for research purposes, then I may be interested in using anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The products we sell, including anti-Tyrosinase Rabbit Monoclonal antibody M00326-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The anti-Tyrosinase Rabbit Monoclonal antibody (M00326-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-23
Question
Can you help my question with product M00326-1, anti-Tyrosinase Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00326-1 anti-Tyrosinase Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-16
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma t-cell using anti-Tyrosinase Rabbit Monoclonal antibody M00326-1. Let me know if you need anything else.
E. Lewis
Verified customer
Asked: 2018-10-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-25
Question
I have attached the WB image, lot number and protocol we used for melanoma t-cell using anti-Tyrosinase Rabbit Monoclonal antibody M00326-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-12
Question
Does anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 work on zebrafish IF with melanoma?
G. Thomas
Verified customer
Asked: 2016-01-12
Answer
Our lab technicians have not tested anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Tyrosinase Rabbit Monoclonal antibody M00326-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish melanoma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-12
