Click image to see more details
Product Info Summary
| SKU: | A00855-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MC1 Receptor/MC1R Antibody Picoband®
View all Melanocortin-1 R/MC1R Antibodies
SKU/Catalog Number
A00855-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MC1 Receptor/MC1R Antibody Picoband® catalog # A00855-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MC1 Receptor/MC1R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00855-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MC1 Receptor.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00855-1 is reactive to MC1R in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
34706 MW
Background of Melanocortin-1 R/MC1R
The melanocortin 1 receptor (MC1R), mapped to 16q24.3, is also known as MSHR. This intronless gene encodes the receptor protein for melanocyte-stimulating hormone (MSH). The encoded protein, a seven pass transmembrane G protein coupled receptor, controls melanogenesis. Two types of melanin exist: red pheomelanin and black eumelanin. Gene mutations that lead to a loss in function are associated with increased pheomelanin production, which leads to lighter skin and hair color. Eumelanin is photoprotective but pheomelanin may contribute to UV-induced skin damage by generating free radicals upon UV radiation. Binding of MSH to its receptor activates the receptor and stimulates eumelanin synthesis. This receptor is a major determining factor in sun sensitivity and is a genetic risk factor for melanoma and non-melanoma skin cancer. Over 30 variant alleles have been identified which correlate with skin and hair color, providing evidence that this gene is an important component in determining normal human pigment variation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00855-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: PC12 whole cell, HEPA1-6 whole cell, HELA whole cell, A375 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MC1 using anti-MC1 antibody (A00855-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: PC12 whole cell lysates,
Lane 2: HEPA1-6 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: A375 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC1 antigen affinity purified polyclonal antibody (Catalog # A00855-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MC1 at approximately 35KD. The expected band size for MC1 is at 35KD.
Protein Target Info & Infographic
Gene/Protein Information For MC1R (Source: Uniprot.org, NCBI)
Gene Name
MC1R
Full Name
Melanocyte-stimulating hormone receptor
Weight
34706 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Melanocyte-stimulating hormone receptor;MSH-R;Melanocortin receptor 1;MC1-R;MC1R;MSHR; MC1R CMM5, MSH-R, SHEP2 melanocortin 1 receptor melanocyte-stimulating hormone receptor|MC1-R|alpha melanocyte stimulating hormone receptor|melanotropin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MC1R, check out the MC1R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MC1R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MC1 Receptor/MC1R Antibody Picoband® (A00855-1)
Hello CJ!
No publications found for A00855-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MC1 Receptor/MC1R Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-MC1 Receptor/MC1R Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-MC1 Receptor/MC1R Antibody Picoband®
Question
I see that the anti-MC1 Receptor/MC1R antibody A00855-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-24
Question
Is this A00855-1 anti-MC1 Receptor/MC1R antibody reactive to the isotypes of MC1R?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The immunogen of A00855-1 anti-MC1 Receptor/MC1R antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MC1 Receptor (290-317aa NAIIDPLIYAFHSQELRRTLKEVLTCSW). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-MC1 Receptor/MC1R antibody A00855-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
The anti-MC1 Receptor/MC1R antibody (A00855-1) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-12
Question
our customer is looking for an antibody suitable for FACS analysis, that binds an extracellular portion of the Melanocortin 1 Receptor (MC1R). Have you got any product suggestions for me in this case?
Verified Customer
Verified customer
Asked: 2017-05-04
Answer
A00855-1 is not able to bind to the extracellular portion of MC1R for the FACS application.
Boster Scientific Support
Answered: 2017-05-04
Question
See attached the WB image, lot number and protocol we used for left lobe of thyroid gland using anti-MC1 Receptor/MC1R antibody A00855-1. Please let me know if you require anything else.
J. Jha
Verified customer
Asked: 2016-08-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-09
Question
When using A00855-1 for Flow Cytometry, is the antibody able to bind to the extracellular portion of MC1R?
Verified Customer
Verified customer
Asked: 2016-07-27
Answer
A00855-1 is not able to bind to the extracellular portion of MC1R.
Boster Scientific Support
Answered: 2016-07-27
Question
Does anti-MC1 Receptor/MC1R antibody A00855-1 work for WB with left lobe of thyroid gland?
A. Jones
Verified customer
Asked: 2015-08-21
Answer
According to the expression profile of left lobe of thyroid gland, MC1R is highly expressed in left lobe of thyroid gland. So, it is likely that anti-MC1 Receptor/MC1R antibody A00855-1 will work for WB with left lobe of thyroid gland.
Boster Scientific Support
Answered: 2015-08-21
