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Product Info Summary
| SKU: | A05397-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tubulin beta Antibody Picoband®
SKU/Catalog Number
A05397-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tubulin beta Antibody Picoband® catalog # A05397-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tubulin beta Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05397-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tubulin beta recombinant protein (Position: A18-D435).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05397-1 is reactive to TUBB1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
39411 MW
Background of TUBB1
TUBB1 is a gene that codes for the protein Tubulin beta-1 chain in humans. This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05397-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human K562 whole cell, human HEL whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue
IHC: human rectal cancer tissue
ICC/IF: U2OS cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tubulin beta using anti-Tubulin beta antibody (A05397-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin beta antigen affinity purified polyclonal antibody (Catalog # A05397-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin beta at approximately 55 kDa. The expected band size for Tubulin beta is at 50 kDa.
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Figure 4. Flow Cytometry analysis of SiHa cells using anti-Tubulin beta antibody (A05397-1).
Overlay histogram showing SiHa cells stained with A05397-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tubulin beta Antibody (A05397-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 2. IHC analysis of Tubulin beta using anti-Tubulin beta antibody (A05397-1).
Tubulin beta was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tubulin beta Antibody (A05397-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IF analysis of Tubulin beta using anti-Tubulin beta antibody (A05397-1).
Tubulin beta was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Tubulin beta Antibody (A05397-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TUBB1 (Source: Uniprot.org, NCBI)
Gene Name
TUBB1
Full Name
Tubulin beta-1 chain
Weight
39411 MW
Superfamily
tubulin family
Alternative Names
RNA binding protein fox-1 homolog 2; Fox-1 homolog B; Hexaribonucleotide-binding protein 2; RNA-binding motif protein 9; RNA-binding protein 9; Repressor of tamoxifen transcriptional activity; RBFOX2; FOX2; HRNBP2; RBM9; RTA TUBB1 tubulin beta 1 class VI tubulin beta-1 chain|tubulin, beta 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB1, check out the TUBB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tubulin beta Antibody Picoband® (A05397-1)
Hello CJ!
A05397-1 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes
The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
Antiproliferative activity of berberine in HepG2 cells via inducing apoptosis and arresting cell cycle
TSPO Deficiency Accelerates Amyloid Pathology and Neuroinflammation by Impairing Microglial Phagocytosis
Circular RNA screening identifies circMYLK4 as a regulator of fast/slow myofibers in porcine skeletal muscles
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973. doi:10.1155/2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
A05397-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973.doi:10.1155/ 2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
A05397-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000
Xu T,Song Q,Zhou L,Yang W,Wu X,Qian Q,Chai H,Han Q,Pan H,Dou X,Li S. Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes. Nutr Metab (Lond)
Species: Mouse
A05397-1 usage in article: APP:WB, SAMPLE:AML-12 CELLS, DILUTION:NA
Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
A05397-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200
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