Product Info Summary
SKU: | A14589-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-TTLL12 Antibody Picoband™
SKU/Catalog Number
A14589-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TTLL12 Antibody Picoband™ catalog # A14589-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TTLL12 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14589-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TTLL12 recombinant protein (Position: E8-Q634).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A14589-1 is reactive to TTLL12 in Human
Applications
A14589-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
64099 MW
Background of TTLL12
Enables H4K20me3 modified histone binding activity and tubulin binding activity. Involved in negative regulation of type I interferon-mediated signaling pathway and regulation of mitotic cell cycle. Located in cytoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a14589 1 ttll12 primary antibodies wb testing 1 a14589 1 ttll12 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a14589-1-ttll12-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of TTLL12 using anti-TTLL12 antibody (A14589-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL12 antigen affinity purified polyclonal antibody (Catalog # A14589-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL12 at approximately 75 kDa. The expected band size for TTLL12 is at 74 kDa.
![a14589 1 ttll12 primary antibodies ihc testing 2 a14589 1 ttll12 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a14589-1-ttll12-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of TTLL12 using anti-TTLL12 antibody (A14589-1).
TTLL12 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL12 Antibody (A14589-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a14589 1 ttll12 primary antibodies fcm testing 4 a14589 1 ttll12 primary antibodies fcm testing 4](https://www.bosterbio.com/media/catalog/product/a/1/a14589-1-ttll12-primary-antibodies-fcm-testing-4.png)
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Figure 4. Flow Cytometry analysis of Caco-2 cells using anti-TTLL12 antibody (A14589-1).
Overlay histogram showing Caco-2 cells stained with A14589-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTLL12 Antibody (A14589-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a14589 1 ttll12 primary antibodies ihc testing 3 a14589 1 ttll12 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a14589-1-ttll12-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of TTLL12 using anti-TTLL12 antibody (A14589-1).
TTLL12 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL12 Antibody (A14589-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a14589 1 ttll12 primary antibodies fcm testing 5 a14589 1 ttll12 primary antibodies fcm testing 5](https://www.bosterbio.com/media/catalog/product/a/1/a14589-1-ttll12-primary-antibodies-fcm-testing-5.png)
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Figure 5. Flow Cytometry analysis of HEL cells using anti-TTLL12 antibody (A14589-1).
Overlay histogram showing HEL cells stained with A14589-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTLL12 Antibody (A14589-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TTLL12 (Source: Uniprot.org, NCBI)
Gene Name
TTLL12
Full Name
Tubulin--tyrosine ligase-like protein 12
Weight
64099 MW
Superfamily
tubulin--tyrosine ligase family
Alternative Names
dJ526I14.2; KIAA0153FLJ41795; tubulin tyrosine ligase-like family, member 12; tubulin--tyrosine ligase-like protein 12 TTLL12 dJ526I14.2 tubulin tyrosine ligase like 12 tubulin--tyrosine ligase-like protein 12|inactive tubulin--tyrosine ligase-like protein 12|tubulin tyrosine ligase-like family, member 12
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTLL12, check out the TTLL12 Infographic
![TTLL12 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTLL12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTLL12 Antibody Picoband™ (A14589-1)
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