Product Info Summary
SKU: | M03258-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2)
View all TIF1 alpha Antibodies
SKU/Catalog Number
M03258-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) catalog # M03258-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03258-2)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
4G6C2
Isotype
Mouse IgG1
Immunogen
E.coli-derived human TRIM24 recombinant protein (Position: A706-D964).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03258-2 is reactive to TRIM24 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
116.831kDa
Background of TIF1 alpha
Tripartite motif-containing 24 (TRIM24) also known as transcriptional intermediary factor 1α (TIF1α) is a protein that, in humans, is encoded by the TRIM24 gene. The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03258-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell
IHC: human adenocarcinoma of the colon tissue, human thyroid cancer tissue, human ovarian serous tumor tissue, human breast cancer tissue
ICC/IF: MCF-7 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TRIM24 using anti-TRIM24 antibody (M03258-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TRIM24 antigen affinity purified monoclonal antibody (Catalog # M03258-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TRIM24 at approximately 130 kDa. The expected band size for TRIM24 is at 117 kDa.
Click image to see more details
Figure 2. IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2).
TRIM24 was detected in a paraffin-embedded section of human adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2).
TRIM24 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2).
TRIM24 was detected in a paraffin-embedded section of human ovarian serous tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of TRIM24 using anti-TRIM24 antibody (M03258-2).
TRIM24 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of TRIM24 using anti-TRIM24 antibody (M03258-2).
TRIM24 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-TRIM24 Antibody (M03258-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TRIM24 (Source: Uniprot.org, NCBI)
Gene Name
TRIM24
Full Name
Transcription intermediary factor 1-alpha
Weight
116.831kDa
Alternative Names
Peptidyl-prolyl cis-trans isomerase B; PPIase B; CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP; PPIB; CYPB TRIM24 PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1 tripartite motif containing 24 transcription intermediary factor 1-alpha|E3 ubiquitin-protein ligase TRIM24|RING finger protein 82|RING-type E3 ubiquitin transferase TIF1-alpha|TIF1-alpha|transcriptional intermediary factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM24, check out the TRIM24 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM24: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2) (M03258-2)
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