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Product Info Summary
| SKU: | RP1100 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRIF/TICAM1 Antibody Picoband®
View all TRIF/TICAM1 Antibodies
SKU/Catalog Number
RP1100
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRIF/TICAM1 Antibody catalog # RP1100. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRIF/TICAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1100)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse TRIF, different from the related human sequence by twelve amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1100 is reactive to Ticam1 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated molecular weight
79230 MW
Background of TRIF/TICAM1
TICAM1 (TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TRIF, is an adapter in responding to activation of toll-like receptors (TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al. (2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al. (2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. Small interfering (si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1100 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Brain Tissue, Mouse Spleen Tissue, Mouse Testis Tissue, Mouse Liver Tissue
Validation Images & Assay Conditions
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Anti-TRIF antibody, RP1100, Western blotting
All lanes: Anti TRIF (RP1100) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: Mouse Spleen Tissue Lysate at 50ug
Lane 3: Mouse Testis Tissue Lysate at 50ug
Lane 4: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 76KD
Observed bind size: 130KD
Protein Target Info & Infographic
Gene/Protein Information For Ticam1 (Source: Uniprot.org, NCBI)
Gene Name
Ticam1
Full Name
TIR domain-containing adapter molecule 1
Weight
79230 MW
Alternative Names
TIR domain-containing adapter molecule 1;TICAM-1;Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta;TIR domain-containing adapter protein inducing IFN-beta;Ticam1;Trif; Ticam1|AW046014, AW547018, TICAM, TICAM-1, TRIF, Tr|toll-like receptor adaptor molecule 1|TIR domain-containing adapter molecule 1|TIR domain-containing adapter protein inducing IFN-beta|TIR domain-containing adaptor inducing interferon-beta|toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ticam1, check out the Ticam1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ticam1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIF/TICAM1 Antibody Picoband® (RP1100)
Hello CJ!
RP1100 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway
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Customer Q&As
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1 Customer Q&As for Anti-TRIF/TICAM1 Antibody Picoband®
Question
We are currently using anti-TRIF/TICAM1 antibody RP1100 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
The anti-TRIF/TICAM1 antibody (RP1100) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-26
