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Product Info Summary
| SKU: | M00102-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-IL-6 Monoclonal Antibody
SKU/Catalog Number
M00102-1
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IL-6 Monoclonal Antibody (Catalog # M00102-1). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store IL 6 antibody at -20°C . For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-6 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00102-1)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 33A12.G9
Isotype
IgG1 kappa
Immunogen
This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Weakly cross-reacts with human PDGF receptor β.
Reactive Species
M00102-1 is reactive to IL6 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
123968 MW
Background of IL-6
IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody is ideal for investigators involved in Cancer, Neuroscience and Immunology research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00102-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000
Flow Cytometry: 0.5 mg/mL
WB: 1:1000
Validation Images & Assay Conditions
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Anti-Human IL-6 Antibody -Flow CytometryHuman PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours.Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly.1mL of permeabilization buffer was added.0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes.1:100 of strep/PE was added and incubated for 30 minutes.LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.
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Flow Cytometry of Human anti-IL-6 antibody M00102-1Cells: human PBMCStimulation: Figure A: un-stimulated, Figure B: 1
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Figure 2. Western blot analysis of IL6 using anti-IL6 antibody (M00102-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # M00102-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for IL6.
Protein Target Info & Infographic
Gene/Protein Information For IL6 (Source: Uniprot.org, NCBI)
Gene Name
IL6
Full Name
Interleukin-6
Weight
123968 MW
Superfamily
IL-6 superfamily
Alternative Names
IL-6, interleukin-6, HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL6, check out the IL6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-6 Monoclonal Antibody (M00102-1)
Hello CJ!
M00102-1 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gang Zhong,Huiping Long,Shiting Ma,Yao Shunhan,Jia Li,Jun Yao,miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis,Life Sciences,Volume 226,2019,Pages 164-172,ISSN 0024-3205,https://doi.org/10.1016/j. lfs.2019.03.071.
Species: Human
M00102-1 usage in article: APP:WB, SAMPLE: CHONDROCYTES, DILUTION:NA
Distribution change of mast cells in human nasal polyps
Serum interleukin-6 is not linked with sleep-quality, restless legs syndrome, and depression, but with 6-month survival in hematological malignancies
Expression of the interleukin 6 system in cortical lesions from patients with tuberous sclerosis complex and focal cortical dysplasia type IIb
Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway
Pannexin3 inhibits TNF???induced inflammatory response by suppressing NF??B signalling pathway in human dental pulp cells
Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity
MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3
Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway
Lu Y, Zhang XS, Zhang ZH, Zhou XM, Gao YY, Liu GJ, Wang H, Wu LY, Li W, Hang CH. J Neuroinflammation. 2018 Mar 19;15(1):87. doi: 10.1186/s12974-018-1118-4. Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarach...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-IL-6 Monoclonal Antibody
Question
I see that the anti-IL-6 Monoclonal antibody M00102-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-17
Question
I was wanting to use your anti-IL-6 Monoclonal antibody for Flow Cytometry for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
D. Collins
Verified customer
Asked: 2019-11-11
Answer
It shows on the product datasheet, M00102-1 anti-IL-6 Monoclonal antibody has been tested for Flow Cytometry, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-11
Question
I am interested in to test anti-IL-6 Monoclonal antibody M00102-1 on human lung for research purposes, then I may be interested in using anti-IL-6 Monoclonal antibody M00102-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The products we sell, including anti-IL-6 Monoclonal antibody M00102-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-10
Question
We have observed staining in human left coronary artery. What should we do? Is anti-IL-6 Monoclonal antibody supposed to stain left coronary artery positively?
A. Carter
Verified customer
Asked: 2019-09-26
Answer
Based on literature left coronary artery does express IL6. Based on Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-26
Question
Is this M00102-1 anti-IL-6 Monoclonal antibody reactive to the isotypes of IL6?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The immunogen of M00102-1 anti-IL-6 Monoclonal antibody is This Protein A purified IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-20
Question
My colleagues were happy with the WB result of your anti-IL-6 Monoclonal antibody. However we have seen positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
Based on literature, lung does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-02-20
Question
See below the WB image, lot number and protocol we used for lung using anti-IL-6 Monoclonal antibody M00102-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-18
Question
Can you help my question with product M00102-1, anti-IL-6 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00102-1 anti-IL-6 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-12
Question
Would M00102-1 anti-IL-6 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
You can see on the product datasheet, M00102-1 anti-IL-6 Monoclonal antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-28
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-IL-6 Monoclonal antibody M00102-1. Let me know if you need anything else.
E. Anderson
Verified customer
Asked: 2017-10-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-16
Question
Is a blocking peptide available for product anti-IL-6 Monoclonal antibody (M00102-1)?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
We do provide the blocking peptide for product anti-IL-6 Monoclonal antibody (M00102-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-11
Question
Does anti-IL-6 Monoclonal antibody M00102-1 work for Flow Cytometry with lung?
V. Rodriguez
Verified customer
Asked: 2016-08-01
Answer
According to the expression profile of lung, IL6 is highly expressed in lung. So, it is likely that anti-IL-6 Monoclonal antibody M00102-1 will work for Flow Cytometry with lung.
Boster Scientific Support
Answered: 2016-08-01
Question
Is there a BSA free version of anti-IL-6 Monoclonal antibody M00102-1 available?
A. Yang
Verified customer
Asked: 2016-02-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-IL-6 Monoclonal antibody M00102-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-24
Question
We ordered your anti-IL-6 Monoclonal antibody for Flow Cytometry on lung in a previous experiment. I am using human, and We want to use the antibody for WB next. I am looking for examining lung as well as fibroblast in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
G. Li
Verified customer
Asked: 2014-03-27
Answer
I took a look at the website and datasheets of our anti-IL-6 Monoclonal antibody and it appears that M00102-1 has been tested on human in both Flow Cytometry and WB. Thus M00102-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-03-27
Question
We are currently using anti-IL-6 Monoclonal antibody M00102-1 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
J. Zhang
Verified customer
Asked: 2013-03-27
Answer
The anti-IL-6 Monoclonal antibody (M00102-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-27
