Anti-TRIAD3A RNF216 Antibody

Product Name

Anti-TRIAD3A RNF216 Antibody

View all TRIAD3 Antibodies

SKU/Catalog Number

A05841

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TRIAD3A RNF216 Antibody (Catalog # A05841). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

TRIAD3A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TRIAD3A RNF216 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05841)

Host

Rabbit

Contents

TRIAD3A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TRIAD3A antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A. The immunogen is located within amino acids 120 - 170 of TRIAD3A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms.

Reactive Species

A05841 is reactive to RNF216 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

99406 MW

Background of TRIAD3

Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05841 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TRIAD3A antibody can be used for detection of TRIAD3A by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For RNF216 (Source: Uniprot.org, NCBI)

Alternative Names

ZIN, CAHH, U7I1, TRIAD3, UBCE7IP1, ZIN, E3 ubiquitin-protein ligase RNF216, RING finger protein 216 RNF216 CAHH, TRIAD3, U7I1, UBCE7IP1, ZIN ring finger protein 216 E3 ubiquitin-protein ligase RNF216|RING-type E3 ubiquitin transferase RNF216|triad domain-containing protein 3|ubiquitin-conjugating enzyme 7-interacting protein 1|zinc finger protein inhibiting NF-kappa-B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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