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Product Info Summary
| SKU: | A02150-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Trem2 Antibody Picoband®
SKU/Catalog Number
A02150-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Trem2 Antibody Picoband® catalog # A02150-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Trem2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02150-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat Trem2 recombinant protein (Position: L19-D131).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02150-3 is reactive to Trem2 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
25.447kDa
Background of Trem2
Triggering receptor expressed on myeloid cells 2 also known as TREM-2 is a protein that in humans is encoded by the TREM2 gene. This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02150-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Rat
ELISA, 0.1-0.5μg/ml,
Positive Control
WB: rat C6 whole cell
FCM: RH35 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Trem2 using anti-Trem2 antibody (A02150-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Trem2 antigen affinity purified polyclonal antibody (Catalog # A02150-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Trem2 at approximately 28KD. The expected band size for Trem2 is at 28KD.
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Figure 2. Flow Cytometry analysis of RH35 cells using anti-Trem2 antibody (A02150-3).
Overlay histogram showing RH35 cells stained with A02150-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Trem2 Antibody (A02150-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Trem2 (Source: Uniprot.org, NCBI)
Gene Name
Trem2
Full Name
Triggering receptor expressed on myeloid cells 2
Weight
25.447kDa
Alternative Names
Mucin-6; MUC-6; Gastric mucin-6; MUC6 TREM2 PLOSL2, TREM-2, Trem2a, Trem2b, Trem2c triggering receptor expressed on myeloid cells 2 triggering receptor expressed on myeloid cells 2|triggering receptor expressed on monocytes 2|triggering receptor expressed on myeloid cells 2a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Trem2, check out the Trem2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Trem2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Trem2 Antibody Picoband® (A02150-3)
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