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Product Info Summary
| SKU: | PB9049 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cathepsin G/CTSG Antibody Picoband®
View all Cathepsin G Antibodies
SKU/Catalog Number
PB9049
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cathepsin G/CTSG Antibody Picoband® catalog # PB9049. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin G/CTSG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9049)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cathepsin G recombinant protein (Position: A76-L255). Human Cathepsin G shares 66% amino acid (aa) sequence identity with mouse Cathepsin G.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9049 is reactive to CTSG in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
28837 MW
Background of Cathepsin G
Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSG gene. This gene is mapped to 14q12, human Cathepsin G gene was expressed in early myeloid precursors in a manner coordinate with the expression of the endogenous murine gene in the bone marrow and spleen. The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9049 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat thymus tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (A04887-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (Catalog # A04887-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 38 kDa. The expected band size for Cathepsin G is at 29 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CTSG (Source: Uniprot.org, NCBI)
Gene Name
CTSG
Full Name
Cathepsin G
Weight
28837 MW
Superfamily
peptidase S1 family
Alternative Names
Cathepsin G;CG;3.4.21.20;CTSG; CTSG CATG, CG cathepsin G cathepsin G
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTSG, check out the CTSG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTSG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cathepsin G/CTSG Antibody Picoband® (PB9049)
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Customer Q&As
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7 Customer Q&As for Anti-Cathepsin G/CTSG Antibody Picoband®
Question
Will anti-Cathepsin G/CTSG antibody PB9049 work for WB with monocyte?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
According to the expression profile of monocyte, CTSG is highly expressed in monocyte. So, it is likely that anti-Cathepsin G/CTSG antibody PB9049 will work for WB with monocyte.
Boster Scientific Support
Answered: 2020-03-04
Question
Will anti-Cathepsin G/CTSG antibody PB9049 work on monkey WB with neutrophil?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Our lab technicians have not validated anti-Cathepsin G/CTSG antibody PB9049 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Cathepsin G/CTSG antibody PB9049 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey neutrophil in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-27
Question
Please see the WB image, lot number and protocol we used for monocyte using anti-Cathepsin G/CTSG antibody PB9049. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-24
Question
Would PB9049 anti-Cathepsin G/CTSG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
It shows on the product datasheet, PB9049 anti-Cathepsin G/CTSG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-16
Question
Can you help my question with product PB9049, anti-Cathepsin G/CTSG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Zhao
Verified customer
Asked: 2016-09-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9049 anti-Cathepsin G/CTSG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-09-02
Question
We are currently using anti-Cathepsin G/CTSG antibody PB9049 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?
J. Jackson
Verified customer
Asked: 2016-04-25
Answer
The anti-Cathepsin G/CTSG antibody (PB9049) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-25
Question
Is this PB9049 anti-Cathepsin G/CTSG antibody reactive to the isotypes of CTSG?
W. Dhar
Verified customer
Asked: 2014-12-19
Answer
The immunogen of PB9049 anti-Cathepsin G/CTSG antibody is E.coli-derived human Cathepsin G recombinant protein (Position: A76-L255). Human Cathepsin G shares 66% amino acid (aa) sequence identity with mouse Cathepsin G. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-12-19
