Anti-Trefoil factor 3 TFF3 Antibody

Product Name

Anti-Trefoil factor 3 TFF3 Antibody

View all TFF3 Antibodies

SKU/Catalog Number

A01738

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Trefoil factor 3 TFF3 Antibody (Catalog # A01738). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TFF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Trefoil factor 3 TFF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01738)

Host

Rabbit

Contents

TFF3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3. The immunogen is located within the last 50 amino acids of TFF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TFF3 antibody is predicted to not cross-react with other TFF family members.

Reactive Species

A01738 is reactive to TFF3 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

10181 MW

Background of TFF3

Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01738 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry at starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For TFF3 (Source: Uniprot.org, NCBI)

Alternative Names

ITF, P1B, TFI, ITF, Trefoil factor 3, Intestinal trefoil factor, hITF TFF3 ITF, P1B, TFI trefoil factor 3 trefoil factor 3|polypeptide P1.B|trefoil factor 3 (intestinal)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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