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Product Info Summary
| SKU: | EK1679 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat TFF3/ITF ELISA Kit PicoKine®
SKU/Catalog Number
EK1679
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat TFF3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Tff3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat TFF3/ITF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1679)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A23-F81
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1679 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1679 is reactive to Tff3 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1679 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TFF3
Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1679-CAP | Anti-Rat Tff3 Pre-coated 96-well strip microplate | 1 |
| EK1679-ST | Rat Tff3 Standard | 2 vials, 10 ng/tube |
| EK1679-DA | Rat Tff3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.051 | 0.120 | 0.197 | 0.339 | 0.624 | 1.048 | 1.658 | 2.089 |
Data Example Images
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Rat TFF3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TFF3, Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat TFF3/ITF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 89 | 213 | 889 | 82 | 218 | 915 |
| Standard deviation | 5.76 | 12.99 | 50.67 | 6.8 | 17.55 | 53.98 |
| CV (%) | 6.7% | 6.1% | 5.7% | 8.3% | 7.5% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TFF3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 89 | 102 | 99 | 102 | 98 | 5.33 | 5.4% |
| Sample 2 | 213 | 209 | 247 | 247 | 229 | 18.05 | 7.8% |
| Sample 3 | 889 | 919 | 932 | 949 | 922 | 21.94 | 2.3% |
Protein Target Info & Infographic
Gene/Protein Information For Tff3 (Source: Uniprot.Org, NCBI)
Gene Name
Tff3
Full Name
Trefoil factor 3
Weight
10.181kDa
Alternative Names
Trefoil factor 3; Intestinal trefoil factor; rITF; Polypeptide P1.B; rP1.B; Tff3; Itf TFF3 ITF, P1B, TFI trefoil factor 3 trefoil factor 3|polypeptide P1.B|trefoil factor 3 (intestinal)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tff3, check out the Tff3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tff3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat TFF3/ITF ELISA Kit PicoKine® (EK1679)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Rat TFF3/ITF ELISA Kit PicoKine®
Question
Does EK1679 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Rat TFF3/ITF ELISA Kit PicoKine® (EK1679) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: is it okay to use citrate plasma as samples in Rat TFF3 Picokine® ELISA Kit (Catalog # EK1679)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TFF3 causing disruption of its protein structure. TFF3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TFF3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-04-01
Question
Q: can you suggest the dilution ratio of serum samples for detection of TFF3 in Rat plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TFF3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-12-26
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-10-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-20
Question
Q: which procedure should I follow in order to thaw whole blood sample for TFF3 ELISA after freezing?
G. Tiwari
Verified customer
Asked: 2018-04-10
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TFF3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-04-10
Question
Q: if the enzyme conjugated TFF3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TFF3 antigen?
Verified Customer
Verified customer
Asked: 2018-03-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-23
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-05-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-05-24
